|Positive WB detected in||Daudi cells, MCF-7 cells, mouse brain tissue, HeLa cells, rat spleen tissue, Jurkat cells, Raji cells, Ramos cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Sample-dependent, check data in validation data gallery|
10600-1-AP targets CD24 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD24 fusion protein Ag0654|
|Full Name||CD24 molecule|
|Calculated molecular weight||8 kDa|
|Observed molecular weight||30-70 kDa|
|GenBank accession number||BC007674|
|Gene ID (NCBI)||100133941|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)
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