CD24 Monoclonal antibody

CD24 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67627-1-Ig
Clone No.1H5C4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

1H5C4, CD24A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite®594
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inMCF-7 cells, Raji cells, Ramos cells, Daudi cells, Jurkat cells, K-562 cells, HL-60 cells
Positive IF/ICC detected inDaudi cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

67627-1-Ig targets CD24 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag11679

Product name: Recombinant human CD24 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-80 aa of BC007674

Sequence: MGRAMVARLGLGLLLLALLLPTQIYSSETTTGTSSNSSQSTSNSGLAPNPTNATTKAAGGALQSTASLFVVSLSLLHLYS

Predict reactive species
Full Name CD24 molecule
Calculated Molecular Weight8 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC007674
Gene Symbol CD24
Gene ID (NCBI) 100133941
RRIDAB_2882828
Conjugate Unconjugated
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP25063
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)

Protocols

Product Specific Protocols
WB protocol for CD24 antibody 67627-1-IgDownload protocol
IF protocol for CD24 antibody 67627-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Breast Cancer (Dove Med Press)

CD24 May Serve as an Immunotherapy Target in Triple-Negative Breast Cancer by Regulating the Expression of PD-L1

Authors - Xudong Zhu
humanWB

Biomedicines

Development of LncRNA Biomarkers in Extracellular Vesicle of Amniotic Fluid Associated with Antenatal Hydronephrosis

Authors - Ying Fu
humanWB

Oncogene

A positive feedback loop of OTUD1 and c-Jun driven by leptin expedites stemness maintenance in ovarian cancer

Authors - Jingtao Wang
Loading...
||
New chat Able
正在加载,请稍候...