CD24 Monoclonal antibody

CD24 Monoclonal Antibody for FC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, FC,ELISA

Conjugate

Unconjugated

CloneNo.

1H5C4

Cat no : 67627-1-Ig

Synonyms

CD24, CD24 molecule, CD24A, FLJ22950, FLJ43543, Signal transducer CD24



Tested Applications

Positive WB detected inMCF-7 cells, Raji cells, Ramos cells, Daudi cells, Jurkat cells, K-562 cells, HL-60 cells
Positive FC detected inRamos cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

67627-1-Ig targets CD24 in WB, FC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD24 fusion protein Ag11679
Full Name CD24 molecule
Calculated molecular weight8 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC007674
Gene symbol CD24
Gene ID (NCBI) 100133941
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)

Protocols

Product Specific Protocols
WB protocol for CD24 antibody 67627-1-IgDownload protocol
FC protocol for CD24 antibody 67627-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols