CD24 Monoclonal antibody
CD24 Monoclonal Antibody for FC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1H5C4
Cat no : 67627-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 cells, Raji cells, Ramos cells, Daudi cells, Jurkat cells, K-562 cells, HL-60 cells |
Positive FC detected in | Ramos cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Sample-dependent, check data in validation data gallery |
Product Information
67627-1-Ig targets CD24 in WB, FC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD24 fusion protein Ag11679 |
Full Name | CD24 molecule |
Calculated molecular weight | 8 kDa |
Observed molecular weight | 42 kDa |
GenBank accession number | BC007674 |
Gene symbol | CD24 |
Gene ID (NCBI) | 100133941 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)
Protocols
Product Specific Protocols | |
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WB protocol for CD24 antibody 67627-1-Ig | Download protocol |
FC protocol for CD24 antibody 67627-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |