CoraLite®594-conjugated CD24 Monoclonal antibody

CD24 Monoclonal Antibody for IF/ICC
Cat No. CL594-67627
Clone No.1H5C4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

CD24A, 1H5C4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IF/ICC detected inDaudi cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-67627 targets CD24 in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag11679

Product name: Recombinant human CD24 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-80 aa of BC007674

Sequence: MGRAMVARLGLGLLLLALLLPTQIYSSETTTGTSSNSSQSTSNSGLAPNPTNATTKAAGGALQSTASLFVVSLSLLHLYS

Predict reactive species
Full Name CD24 molecule
Calculated Molecular Weight8 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC007674
Gene Symbol CD24
Gene ID (NCBI) 100133941
RRIDAB_2920165
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP25063
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)

Protocols

Product Specific Protocols
IF protocol for CL594 CD24 antibody CL594-67627Download protocol
Standard Protocols
Click here to view our Standard Protocols
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