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CoraLite®594-conjugated CD24 Monoclonal antibody

CD24 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H5C4

Cat no : CL594-67627

Synonyms

CD24, CD24 molecule, CD24A, FLJ22950, FLJ43543, Signal transducer CD24

Validation Data Gallery

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  • Flow cytometry (FC) experiment of Ramos cells using CoraLite®594-conjugated CD24 Monoclonal antibody (CL594-67627)

    FC experiment of Ramos using CL594-67627

    1X10^6 Ramos cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CD24 (CL594-67627, Clone:1H5C4) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inRamos cells

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

CL594-67627 targets CD24 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD24 fusion protein Ag11679
Full Name CD24 molecule
Calculated molecular weight8 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC007674
Gene symbol CD24
Gene ID (NCBI) 100133941
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)

Protocols

Product Specific Protocols
FC protocol for CL594 CD24 antibody CL594-67627Download protocol
Standard Protocols
Click here to view our Standard Protocols