CD300f Recombinant monoclonal antibody, PBS Only

• 

  WB analysis of U-937 using 87497-1-RR (same clone as 87497-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 87497-1-RR (CD300f antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87497-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 87497-1-RR (same clone as 87497-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 87497-1-RR (CD300f antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87497-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 87497-1-RR (same clone as 87497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87497-1-RR (CD300f antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87497-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 87497-1-RR (same clone as 87497-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87497-1-RR (CD300f antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87497-1-PBS in a different storage buffer formulation.

CD300f Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 87497-1-PBS

Clone No.252756G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

CD300LF, CD300 antigen-like family member F, CLM 1, CLM1, CLM-1

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Product Information

87497-1-PBS targets CD300f in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg10440

  Product name: Recombinant human CD300F protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 10011473 of

  Sequence: \

• Full Name: CD300 molecule-like family member f
• Observed Molecular Weight: 40-60 kDa
• Gene Symbol: CD300LF
• Gene ID (NCBI): 146722
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8TDQ1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD300f (also known as CLM-1 or IREM-1) is a type I transmembrane glycoprotein belonging to the CD300 family of immunoreceptors. Expressed predominantly on myeloid cells, including macrophages, dendritic cells, and mast cells, CD300f functions as an inhibitory receptor that regulates immune responses. Its extracellular domain contains a single IgV-like domain that recognizes phosphatidylserine and phosphatidylethanolamine, enabling recognition of apoptotic cells. The cytoplasmic tail harbors immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that recruit phosphatases SHP-1 and SHP-2 upon phosphorylation, delivering negative signals. CD300f plays critical roles in dampening inflammation, promoting efferocytosis, and maintaining immune homeostasis (PMID: 28073916, PMID: 33548578, PMID: 37302321). The predicted molecular weight of CD300f is 32 kDa; however, due to different levels of N- and/or O-linked glycosylation modifications, its molecular weight may range from 40 to 60 kDa (PMID: 15549731, PMID: 31338922).