CD300f Recombinant monoclonal antibody

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  WB analysis of U-937 using 87497-1-RR

  U-937 cells were subjected to SDS PAGE followed by western blot with 87497-1-RR (CD300f antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 87497-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87497-1-RR (CD300f antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 87497-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87497-1-RR (CD300f antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 87497-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 87497-1-RR (CD300f antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD300f Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 87497-1-RR

Clone No.252756G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CD300LF, CD300 antigen-like family member F, CLM 1, CLM1, CLM-1

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Tested Applications

• Positive WB detected in: U-937 cells, HL-60 cells, THP-1 cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87497-1-RR targets CD300f in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg10440

  Product name: Recombinant human CD300F protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 10011473 of

  Sequence: \

• Full Name: CD300 molecule-like family member f
• Observed Molecular Weight: 40-60 kDa
• Gene Symbol: CD300LF
• Gene ID (NCBI): 146722
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8TDQ1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD300f (also known as CLM-1 or IREM-1) is a type I transmembrane glycoprotein belonging to the CD300 family of immunoreceptors. Expressed predominantly on myeloid cells, including macrophages, dendritic cells, and mast cells, CD300f functions as an inhibitory receptor that regulates immune responses. Its extracellular domain contains a single IgV-like domain that recognizes phosphatidylserine and phosphatidylethanolamine, enabling recognition of apoptotic cells. The cytoplasmic tail harbors immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that recruit phosphatases SHP-1 and SHP-2 upon phosphorylation, delivering negative signals. CD300f plays critical roles in dampening inflammation, promoting efferocytosis, and maintaining immune homeostasis (PMID: 28073916, PMID: 33548578, PMID: 37302321). The predicted molecular weight of CD300f is 32 kDa; however, due to different levels of N- and/or O-linked glycosylation modifications, its molecular weight may range from 40 to 60 kDa (PMID: 15549731, PMID: 31338922).