CoraLite®488-conjugated CD31 Monoclonal antibody

CD31 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

2A1E2

Cat no : CL488-66065

Synonyms

CD31, EndoCAM, FLJ58394, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker



Tested Applications

Positive IF detected inLymphatic endothelial cells cells, human tonsillitis tissue
For other applications, we recommend the unconjugated version of this antibody, 66065-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL488-66065 is CD31 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD31 fusion protein Ag1787
Full Name platelet/endothelial cell adhesion molecule
Calculated molecular weight 83 kDa
GenBank accession numberBC022512
Gene symbol PECAM1
Gene ID (NCBI) 5175
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489). This antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
IF protocol for CL488 CD31 antibody CL488-66065Download protocol
Standard Protocols
Click here to view our Standard Protocols