CoraLite®594-conjugated CD31 Monoclonal antibody

CD31 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3F8E2

Cat no : CL594-66065

Synonyms

CD31, EndoCAM, FLJ58394, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker



Tested Applications

Positive IF detected inhuman tonsillitis tissue, human placenta tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

CL594-66065 targets CD31 in WB, IF applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD31 fusion protein Ag1787
Full Name platelet/endothelial cell adhesion molecule
Calculated Molecular Weight 83 kDa
GenBank Accession NumberBC022512
Gene Symbol PECAM1
Gene ID (NCBI) 5175
RRIDAB_2883500
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).This antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

Protocols

Product Specific Protocols
IF protocol for CL594 CD31 antibody CL594-66065Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

Targeting Erbin-mitochondria axis in platelets/megakaryocytes promotes B cell-mediated antitumor immunity

Authors - Zilong Zhang