Anti-Mouse CD357 (GITR) (DTA-1)

CD357 (GITR) Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

DTA-1

Cat no : 65102-1-Ig

Synonyms

CD357, Tnfrsf18

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Validation Data Gallery

• 

  FC experiment of mouse splenocytes using 65102-1-Ig

  1x10^6 mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD357 (GITR) (65102-1-Ig, Clone:DTA-1) or 0.5 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2) and FITC anti-rat IgG2b Antibody at dilution 1:100. Cells were co-stained with CoraLite 647 Anti-Human CD25. CD4+ cells were gated. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65102-1-Ig targets CD357 (GITR) in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse CD25+ CD4+ T cells
• Full Name: tumor necrosis factor receptor superfamily, member 18
• GenBank Accession Number: BC146517
• Gene Symbol: Tnfrsf18
• Gene ID (NCBI): 21936
• RRID: AB_2918402
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Glucocorticoid-induced TNFR-related protein (GITR), also known as CD357 or TNFRSF18, is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is expressed constitutively at high levels in T regulatory cells (Treg cells) and plays a key role in dominant immunological self-tolerance maintained by CD25+CD4+ regulatory T cells (PMID: 11812990). It is expressed at low levels on resting responder T cells. The expression of GITR on T cells can be upregulated upon activation (PMID: 15770698). GITR is activated by GITR ligand (GITRL) which is mainly expressed on APC. GITR-GITRL interactions could co-stimulate both responder T-cell functions and the suppressive functions of Treg cells (PMID: 16868552).