CoraLite® Plus 488 Anti-Mouse GITR/TNFRSF18 (DTA-1)

• 

  FC experiment of mouse splenocytes using CL488-65102

  1X10^6 mouse splenocytes were surface stained with PE-Anti-Mouse CD4 (PE-65104, Clone: GK1.5), CoraLite® Plus 647-Conjugated Anti-Mouse CD25 (CL647-65137, Clone: PC61.5) and 0.5 ug CoraLite® Plus 488 Anti-Mouse CD357 (GITR) (CL488-65102, Clone: DTA-1). Cells were not fixed. CD4+ lymphocytes were gated for analysis of CD25 and CD357 (GITR) staining.

GITR/TNFRSF18 Monoclonal Antibody for FC

Cat No. CL488-65102

Clone No.DTA-1

Host / Isotype

Rat / IgG2b, kappa

Reactivity

mouse

Applications

FC

Synonyms

CD357, GITR, Tnfrsf18, AITR, Glucocorticoid-induced TNFR-related protein

---

Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65102 targets GITR/TNFRSF18 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Mouse CD25+ CD4+ T cells

• Full Name: tumor necrosis factor receptor superfamily, member 18
• GenBank Accession Number: BC146517
• Gene Symbol: CD357
• Gene ID (NCBI): 21936
• RRID: AB_2883176
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: O35714
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Glucocorticoid-induced TNFR-related protein (GITR), also known as CD357 or TNFRSF18, is a member of the tumor necrosis factor receptor (TNF-R) superfamily. GITR is expressed constitutively at high levels in T regulatory cells (Treg cells) and plays a key role in dominant immunological self-tolerance maintained by CD25+CD4+ regulatory T cells (PMID: 11812990). It is expressed at low levels on resting responder T cells. The expression of GITR on T cells can be upregulated upon activation (PMID: 15770698). GITR is activated by GITR ligand (GITRL) which is mainly expressed on APC. GITR-GITRL interactions could co-stimulate both responder T-cell functions and the suppressive functions of Treg cells (PMID: 16868552).