Anti-Human CD367/CLEC4A Rabbit Recombinant Antibody

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• Anti-Human CD367/CLEC4A Rabbit Recombinant Antibody (98230-2-RR)

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  FC experiment of human peripheral blood lymphocytes using 98230-2-RR

  1x10^6 human peripheral blood lymphocytes were surface stained with 0.25 ug Anti-Human CD367/CLEC4A Rabbit RecAb (98230-2-RR, Clone: 241854D7) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human peripheral blood lymphocytes using 98230-2-RR

  1x10^6 human peripheral blood lymphocytes were surface stained with 0.25 ug Anti-Human CD367/CLEC4A Rabbit RecAb (98230-2-RR, Clone: 241854D7) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

• 

  Affinity of 98230-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98230-2-RR against Human CD367/CLEC4A were performed. The affinity constant is 0.70 nM.

CD367/CLEC4A Uni-rAb^® Recombinant Antibody for FC

Cat No. 98230-2-RR

Clone No.241854D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

CLEC4A, CD367, CLECSF6, DCIR, DDB27

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Tested Applications

• Positive FC detected in: human peripheral blood lymphocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98230-2-RR targets CD367/CLEC4A in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1350

  Product name: Recombinant Human DCIR/CLEC4A protein (rFc Tag)(HPLC verified)

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 70-237 aa of NM_016184.4

  Sequence: QKYSQLLEKKTTKELVHTTLECVKKNMPVEETAWSCCPKNWKSFSSNCYFISTESASWQDSEKDCARMEAHLLVINTQEEQDFIFQNLQEESAYFVGLSDPEGQRHWQWVDQTPYNESSTFWHPREPSDPNERCVVLNFRKSPKRWGWNDVNCLGPQRSVCEMMKIHL

• Full Name: C-type lectin domain family 4, member A
• Calculated Molecular Weight: 28 kDa
• GenBank Accession Number: NM_016184.4
• Gene Symbol: CLEC4A
• Gene ID (NCBI): 50856
• RRID: AB_3745897
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UMR7-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD367 (CLEC4A/DCIR) is a C-type lectin receptor primarily expressed on antigen-presenting cells. CD367 features a characteristic C-type lectin domain (CRD) in its extracellular region that recognizes carbohydrate ligands in a calcium-dependent manner and exhibits a restricted hematopoietic expression profile particularly useful for dendritic cell (DC) subset identification.