CD4 Monoclonal antibody

CD4 Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (4)

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2H4A2

Cat no : 67786-1-Ig

Synonyms

CD4, CD4 molecule, CD4mut



Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human colon tissue, human liver tissue, human thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:450-1:1800
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67786-1-Ig targets CD4 in IHC, IF, FC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD4 fusion protein Ag30870
Full Name CD4 molecule
Calculated Molecular Weight 55 kDa
GenBank Accession NumberBC025782
Gene Symbol CD4
Gene ID (NCBI) 920
RRIDAB_2918550
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
IHC protocol for CD4 antibody 67786-1-IgDownload protocol
IF protocol for CD4 antibody 67786-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF

J Med Virol

Combination of novel oncolytic herpesvirus with paclitaxel as an efficient strategy for breast cancer therapy

Authors - Xinyue Deng
mouseIF

Cardiovasc Res

Inhibition of PGK1 attenuates autoimmune myocarditis by reprogramming CD4+ T cells metabolism

Authors - Yang Lu
humanIHC

Clin Transl Med

Single-cell transcriptome sequencing of B-cell heterogeneity and tertiary lymphoid structure predicts breast cancer prognosis and neoadjuvant therapy efficacy

Authors - Qing Wang
human,mouseIHC

Front Immunol

LRPPRC facilitates tumor progression and immune evasion through upregulation of m6A modification of PD-L1 mRNA in hepatocellular carcinoma

Authors - Houhong Wang
humanIHC

Front Immunol

Characteristics of the immunogenicity and tumor immune microenvironment in HER2-amplified lung adenocarcinoma

Authors - Qinyang Wang
mouseIF

Immunology

Deubiquitinase inhibitor PR-619 potentiates colon cancer immunotherapy by inducing ferroptosis

Authors - Jingjing Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Emma (Verified Customer) (11-29-2021)

Used for IF on FFPE prostate tissue. Antigen retrieval Tris-EDTA pH 9.0.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Prostate