CD4 Monoclonal antibody

CD4 Monoclonal Antibody for IF, IHC,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

IHC, IF

Conjugate

Unconjugated

CloneNo.

2H4A2

Cat no : 67786-1-Ig

Synonyms

CD4, CD4 molecule, CD4mut



Tested Applications

Positive IHC detected inhuman tonsillitis tissue, human colon tissue, human liver tissue, human thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:450-1:1800
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 3 publications below

Product Information

67786-1-Ig targets CD4 in IHC, IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD4 fusion protein Ag30870
Full Name CD4 molecule
Calculated molecular weight 55 kDa
GenBank accession numberBC025782
Gene symbol CD4
Gene ID (NCBI) 920
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Protocols

Product Specific Protocols
IHC protocol for CD4 antibody 67786-1-IgDownload protocol
IF protocol for CD4 antibody 67786-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Chin Med J (Engl)

Hyperbaric oxygen treatment on keloid tumor immune gene expression.

Authors - Chun-Hu Wang
humanIHC

Gynecol Oncol

Increased co-expression of MEST and BRCA1 is associated with worse prognosis and immune infiltration in ovarian cancer.

Authors - Jing Zhang
humanIHC

Front Oncol

A Novel Strategy Conjugating PD-L1 Polypeptide With Doxorubicin Alleviates Chemotherapeutic Resistance and Enhances Immune Response in Colon Cancer.

Authors - Maolin Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Emma (Verified Customer) (11-29-2021)

Used for IF on FFPE prostate tissue. Antigen retrieval Tris-EDTA pH 9.0.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Prostate