Validation Data Gallery
|Positive IHC detected in||human tonsillitis tissue, human colon tissue, human liver tissue, human thymus tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue|
|Immunohistochemistry (IHC)||IHC : 1:450-1:1800|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
67786-1-Ig targets CD4 in IHC, IF, ELISA applications and shows reactivity with Human samples.
|Cited Reactivity||human, rat, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||CD4 fusion protein Ag30870|
|Full Name||CD4 molecule|
|Calculated molecular weight||55 kDa|
|GenBank accession number||BC025782|
|Gene ID (NCBI)||920|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
Characteristics of the immunogenicity and tumor immune microenvironment in HER2-amplified lung adenocarcinoma
The Immunological Contribution of a Novel Metabolism-Related Signature to the Prognosis and Anti-Tumor Immunity in Cervical Cancer.
Chronic stress-induced depression requires the recruitment of peripheral Th17 cells into the brain.
Immunity and Extracellular Matrix Characteristics of Breast Cancer Subtypes Based on Identification by T Helper Cells Profiling.
A Novel Strategy Conjugating PD-L1 Polypeptide With Doxorubicin Alleviates Chemotherapeutic Resistance and Enhances Immune Response in Colon Cancer.
Increased co-expression of MEST and BRCA1 is associated with worse prognosis and immune infiltration in ovarian cancer.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Emma (Verified Customer) (11-29-2021)
Used for IF on FFPE prostate tissue. Antigen retrieval Tris-EDTA pH 9.0.