CD4 Monoclonal antibody
CD4 Monoclonal Antibody for IF, IHC,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (4)
Applications
IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
2H4A2
Cat no : 67786-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human tonsillitis tissue, human colon tissue, human liver tissue, human thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human tonsillitis tissue |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:450-1:1800 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
IHC | See 12 publications below |
IF | See 13 publications below |
FC | See 1 publications below |
Product Information
67786-1-Ig targets CD4 in IHC, IF, FC, ELISA applications and shows reactivity with Human samples.
Tested Reactivity | Human |
Cited Reactivity | human, mouse, rat, pig |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD4 fusion protein Ag30870 |
Full Name | CD4 molecule |
Calculated molecular weight | 55 kDa |
GenBank accession number | BC025782 |
Gene symbol | CD4 |
Gene ID (NCBI) | 920 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
Protocols
Product Specific Protocols | |
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IHC protocol for CD4 antibody 67786-1-Ig | Download protocol |
IF protocol for CD4 antibody 67786-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Med Virol Combination of novel oncolytic herpesvirus with paclitaxel as an efficient strategy for breast cancer therapy | ||
Cardiovasc Res Inhibition of PGK1 attenuates autoimmune myocarditis by reprogramming CD4+ T cells metabolism | ||
Clin Transl Med Single-cell transcriptome sequencing of B-cell heterogeneity and tertiary lymphoid structure predicts breast cancer prognosis and neoadjuvant therapy efficacy | ||
Front Immunol LRPPRC facilitates tumor progression and immune evasion through upregulation of m6A modification of PD-L1 mRNA in hepatocellular carcinoma | ||
Front Immunol Characteristics of the immunogenicity and tumor immune microenvironment in HER2-amplified lung adenocarcinoma | ||
Immunology Deubiquitinase inhibitor PR-619 potentiates colon cancer immunotherapy by inducing ferroptosis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Emma (Verified Customer) (11-29-2021) | Used for IF on FFPE prostate tissue. Antigen retrieval Tris-EDTA pH 9.0.
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