Validation Data Gallery
|Positive IHC detected in||human tonsillitis tissue, human colon tissue, human liver tissue, human thymus tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue|
|Immunohistochemistry (IHC)||IHC : 1:450-1:1800|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
67786-1-Ig targets CD4 in IHC, IF, FC, ELISA applications and shows reactivity with Human samples.
|Cited Reactivity||human, mouse, rat, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||CD4 fusion protein Ag30870|
|Full Name||CD4 molecule|
|Calculated molecular weight||55 kDa|
|GenBank accession number||BC025782|
|Gene ID (NCBI)||920|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Emma (Verified Customer) (11-29-2021)
Used for IF on FFPE prostate tissue. Antigen retrieval Tris-EDTA pH 9.0.