CD4 Monoclonal antibody

CD4 Monoclonal Antibody for IF, IHC,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (4)

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

26

CloneNo.

2H4A2

Reviews (1)

Cat no : 67786-1-Ig

Synonyms

CD4, CD4 molecule, CD4mut

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Validation Data Gallery

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  IHC staining of human tonsillitis using 67786-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67786-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thymus using 67786-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thymus using 67786-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 67786-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 67786-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67786-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67786-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 67786-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD4 antibody (67786-1-Ig, Clone: 2H4A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue, human colon tissue, human liver tissue, human thymus tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human tonsillitis tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:450-1:1800
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• IHC: See 12 publications below
• IF: See 13 publications below
• FC: See 1 publications below

Product Information

67786-1-Ig targets CD4 in IHC, IF, FC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD4 fusion protein Ag30870
• Full Name: CD4 molecule
• Calculated molecular weight: 55 kDa
• GenBank accession number: BC025782
• Gene symbol: CD4
• Gene ID (NCBI): 920
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Publications

Species	Application	Title
human,mouse	IF	J Med Virol; Combination of novel oncolytic herpesvirus with paclitaxel as an efficient strategy for breast cancer therapy; Authors - Xinyue Deng
mouse	IF	Cardiovasc Res; Inhibition of PGK1 attenuates autoimmune myocarditis by reprogramming CD4+ T cells metabolism; Authors - Yang Lu
human	IHC	Clin Transl Med; Single-cell transcriptome sequencing of B-cell heterogeneity and tertiary lymphoid structure predicts breast cancer prognosis and neoadjuvant therapy efficacy; Authors - Qing Wang
human,mouse	IHC	Front Immunol; LRPPRC facilitates tumor progression and immune evasion through upregulation of m6A modification of PD-L1 mRNA in hepatocellular carcinoma; Authors - Houhong Wang
human	IHC	Front Immunol; Characteristics of the immunogenicity and tumor immune microenvironment in HER2-amplified lung adenocarcinoma; Authors - Qinyang Wang
mouse	IF	Immunology; Deubiquitinase inhibitor PR-619 potentiates colon cancer immunotherapy by inducing ferroptosis; Authors - Jingjing Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Emma (Verified Customer) (11-29-2021)

Used for IF on FFPE prostate tissue. Antigen retrieval Tris-EDTA pH 9.0.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate