Filter: AllIHCIF-P

IHC staining of human tonsillitis using 67786-1-Ig (same clone as 67786-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 67786-1-Ig (same clone as 67786-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 67786-1-Ig (same clone as 67786-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 67786-1-Ig (same clone as 67786-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 67786-1-Ig (same clone as 67786-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.
IHC staining of human thymus using 67786-1-Ig (same clone as 67786-1-PBS)
Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.
IHC staining of human thymus using 67786-1-Ig (same clone as 67786-1-PBS)
Immunohistochemical analysis of paraffin-embedded human thymus tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 67786-1-Ig (same clone as 67786-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67786-1-Ig (CD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.
IF Staining of human tonsillitis using 67786-1-Ig (same clone as 67786-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD4 antibody (67786-1-Ig, Clone: 2H4A2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67786-1-PBS in a different storage buffer formulation.

CD4 Monoclonal antibody, PBS Only

CD4 Monoclonal Antibody for IHC, IF-P, Indirect ELISA

Cat No. 67786-1-PBS

Clone No.2H4A2

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, IF-P, Indirect ELISA

Synonyms

2H4A2, CD4 molecule, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67786-1-PBS targets CD4 in IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag30870 Product name: Recombinant human CD4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 26-226 aa of BC025782 Sequence: KKVVLGKKGDTVELTCTASQKKSIQFHWKNSNQIKILGNQGSFLTKGPSKLNDRADSRRSLWDQGNFPLIIKNLKIEDSDTYICEVEDQKEEVQLLVFGLTANSDTHLLQGQSLTLTLESPPGSSPSVQCRSPRGKNIQGGKTLSVSQLELQDSGTWTCTVLQNQKKVEFKIDIVVLAFQKASSIVYKKEGEQVEFSFPLA Predict reactive species
Full Name	CD4 molecule
Calculated Molecular Weight	55 kDa
GenBank Accession Number	BC025782
Gene Symbol	CD4
Gene ID (NCBI)	920
ENSEMBL Gene ID	ENSG00000010610
RRID	AB_2918550
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P01730
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).