Anti-Mouse CD41 (MWReg30)

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  FC experiment of mouse peripheral blood platelets using 65189-1-Ig

  1x10^6 mouse peripheral blood platelets were surface stained with 0.5 ug Anti-Mouse CD41 (MWReg30) (65189-1-Ig, Clone: MWReg30) (red) or Rat IgG1 Isotype Control (HRPN) (65212-1-Ig, Clone: HRPN) (blue), and FITC Anti-Rat IgG1 antibody. Cells were not fixed.

CD41 Monoclonal Antibody for FC

Cat No. 65189-1-Ig

Clone No.MWReg30

Host / Isotype

Rat / IgG1, kappa

Reactivity

mouse

Applications

FC

Synonyms

Itga2b, GpIIb, integrin alpha 2b, MWReg30

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Tested Applications

• Positive FC detected in: mouse peripheral blood platelets

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65189-1-Ig targets CD41 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Mouse platelets

• Full Name: integrin alpha 2b
• GenBank Accession Number: BC120493
• Gene Symbol: CD41
• Gene ID (NCBI): 16399
• RRID: AB_3084902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9QUM0
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID: 19118207). Integrin alpha-IIb (ITGA2B, CD41) is expressed on platelets, megakaryocytes and some hematopoietic progenitor cells (PMID: 11934866). This protein undergoes post-translational cleavage to yield disulfide linked light beta (25 kDa) and heavy alpha (125 kDa) chains, which join with integrin beta3 (CD61) to form a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. CD41/CD61 is crucial for platelet aggregation through binding of soluble fibrinogen.