CD43 Recombinant monoclonal antibody

• 

  WB analysis of rat thymus using 86985-1-RR

  rat thymus tissue was subjected to SDS PAGE followed by western blot with 86985-1-RR (CD43 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 86985-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86985-1-RR against Rat CD43 were performed. The affinity constant is 0.256 nM.

CD43 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86985-1-RR

Clone No.251932G11

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, ELISA

Synonyms

Spn, gpL115, Lsn, Lsn1, sialophorin

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Tested Applications

• Positive WB detected in: rat thymus tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86985-1-RR targets CD43 in WB, ELISA applications and shows reactivity with rat samples.

• Tested Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg5149

  Product name: recombinant rat CD43 protein

  Source: mammalian cells-derived, pHZ-KIsec-N-rFc

  Tag: N-rFc

  Domain: 8-231 aa of P13838

  Sequence: ENLPNTMTMLPFTPNSESPSTSEALSTYSSIATVPVTEDPKESISPWGQTTAPASSIPLGTPELSSFFFTSAGASGNTPVPELTTSQEVSTEASLVLFPKSSGVASDPPVTITNPATSSAVASTSLETFKGTSAPPVTVTSSTMTSGPFVATTVSSETSGPPVTMATGSLGPSKETHGLSATIATSSGESSSVAGGTPVFSTKISTTSTPNPITTVPPRPGSSG

• Full Name: sialophorin
• Calculated Molecular Weight: 38 kDa
• Observed Molecular Weight: 90-110 kDa
• GenBank Accession Number: P13838
• Gene Symbol: CD43
• Gene ID (NCBI): 24796
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13838
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD43, also known as leukosialin, sialophorin, leukocyte sialoglycoprotein, SPN and W3/13 antigen, is a mucin-like type I transmembrane protein. Rat CD43 is a sialoglycoprotein with an apparent molecular weight of ~100 kDa and is found on thymocytes, T lymphocytes, neutrophils, plasma cells and myelomas but not on B lymphocytes or most other tissues (PMID: 2965006). CD43 is involved in the contact between cells participating in a series of events such as signaling, adherence, and host-parasite interactions (PMID: 10580634; 31197200).