CD68 Polyclonal antibody

CD68 Polyclonal Antibody for FC, IF, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 25747-1-AP

Synonyms



Tested Applications

Positive IP detected inRAW 264.7 cells
Positive IHC detected inhuman liver tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Positive FC detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25747-1-AP is CD68 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, canine, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD68 fusion protein Ag22815
Full Name CD68 molecule
Calculated molecular weight 37 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC015557
Gene symbol CD68
Gene ID (NCBI) 968
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages. The apparent molecular weight of CD68 is larger than calculated molecular weight due to post-translation modification.

Protocols

Product Specific Protocols
IHC protocol for CD68 antibody 25747-1-APDownload protocol
IF protocol for CD68 antibody 25747-1-APDownload protocol
IP protocol for CD68 antibody 25747-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Metab

Pharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species.

Authors - Xiao-Jing Zhang
mouseIHC

Nat Commun

The ubiquitin ligase ZNRF1 promotes caveolin-1 ubiquitination and degradation to modulate inflammation.

Authors - Chih-Yuan Lee
canineIF

Curr Biol

Sequential oncogenic mutations influence cell competition.

Authors - Koki Kohashi
mouseIF

Theranostics

Mitochondrial STAT3 exacerbates LPS-induced sepsis by driving CPT1a-mediated fatty acid oxidation.

Authors - Rongqing Li
humanWB, IF

Biomaterials

Attempted caveolae-mediated phagocytosis of surface-fixed micro-pillars by human osteoblasts.

Authors - Caroline Moerke
ratIF

Theranostics

A biocompatible vascularized graphene oxide (GO)-collagen chamber with osteoinductive and anti-fibrosis effects promotes bone regeneration in vivo.

Authors - Huimin Fang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue with a Tris-EDTA antigen retrieval. Also works by IHC.

  • Applications: Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Prostate
CD68 Antibody Immunohistochemistry,Immunofluorescence validation (1:200 dilution) in Prostate (Cat no:25747-1-AP)
FH

Fabio Henrique (Verified Customer) (01-17-2019)

Figure 1. IF of CD68 (red) in human spleen. Formalin-fixed paraffin-embedded human spleen tissue was used as positive control to probe for CD68. Heat-induced antigen retrieval was performed in sodium citrate buffer pH 6.0 + Tween20 at 0.5%. Tissue incubated at 96*C for 20 min inside the buffer. Permeabilization was done with washes of TBSTritonX 0.25% for 3x 5min and blocking was done in TBS 10% Goat serum, 1% BSA, for 1 hr at RT. Anti-CD68 was used at 1:200 in blocking buffer, overnight incubation at 4*C. Secondary antibody AlexaFluor 488 was used at 1:500 for 1 hr at RT. Imaged using a Nikon A1 confocal microscope. Figure 2: CD68 (red) and CD206 (green) in human primary macrophages polarized to M2-phenotype, encapsulated in 3D hydrogel (hyaluronic acid and collagen type 1). Staining was performed as described above, except primary (CD68) was used at 1:100 and secondary antibodies were used at 1:500.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100 (hydrogels); 1:200 (2D samples)
  • Cell Tissue Type: Spleen and 3D culture of human primary macrophages
CD68 Antibody Immunofluorescence, validation (1:100 (hydrogels); 1:200 (2D samples) dilution) in Spleen and 3D culture of human primary macrophages (Cat no:25747-1-AP)