Validation Data Gallery
|Positive IP detected in||RAW 264.7 cells|
|Positive IHC detected in||human liver tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue|
|Positive FC detected in||RAW 264.7 cells|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:2000-1:8000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 25747-1-AP is CD68 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, canine, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD68 fusion protein Ag22815|
|Full Name||CD68 molecule|
|Calculated molecular weight||37 kDa|
|Observed molecular weight||60 kDa|
|GenBank accession number||BC015557|
|Gene ID (NCBI)||968|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages. The apparent molecular weight of CD68 is larger than calculated molecular weight due to post-translation modification.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Emma (Verified Customer) (11-29-2021)
Works well by IF on FFPE tissue with a Tris-EDTA antigen retrieval. Also works by IHC.
Fabio Henrique (Verified Customer) (01-17-2019)
Figure 1. IF of CD68 (red) in human spleen. Formalin-fixed paraffin-embedded human spleen tissue was used as positive control to probe for CD68. Heat-induced antigen retrieval was performed in sodium citrate buffer pH 6.0 + Tween20 at 0.5%. Tissue incubated at 96*C for 20 min inside the buffer. Permeabilization was done with washes of TBSTritonX 0.25% for 3x 5min and blocking was done in TBS 10% Goat serum, 1% BSA, for 1 hr at RT. Anti-CD68 was used at 1:200 in blocking buffer, overnight incubation at 4*C. Secondary antibody AlexaFluor 488 was used at 1:500 for 1 hr at RT. Imaged using a Nikon A1 confocal microscope. Figure 2: CD68 (red) and CD206 (green) in human primary macrophages polarized to M2-phenotype, encapsulated in 3D hydrogel (hyaluronic acid and collagen type 1). Staining was performed as described above, except primary (CD68) was used at 1:100 and secondary antibodies were used at 1:500.