Anti-Human CD68 (Y1/82A)

• 

  IF Staining of THP-1 using 65187-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD68 antibody (65187-1-Ig, Clone: Y1/82A ) at dilution of 1:400 and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Mouse IgG (H+L), CoraLite® Plus 555 CD45 antibody (CL555-65109, Clone: HI30).

• 

  FC experiment of human PBMCs using 65187-1-Ig

  1X10^6 human PBMCs were intracellularly stained with 0.2 ug Anti-Human CD68 (65187-1-Ig, Clone:Y1/82A) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Monocytes were gated.

CD68 Monoclonal Antibody for IF/ICC, FC (Intra)

Cat No. 65187-1-Ig

Clone No.Y1/82A

Publications(9)

Host / Isotype

Mouse / IgG2b, kappa

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra)

Synonyms

CD68 molecule, GP110, Macrosialin, SCARD1, Y1/82A

---

Tested Applications

• Positive IF/ICC detected in: THP-1 cells
• Positive FC (Intra) detected in: human PBMCs
• Positive FC detected in: human PBMCs

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.2 ug per 10^6 cells in 100 μl suspension
• Flow Cytometry (FC): FC : 0.2 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 6 publications below

Product Information

65187-1-Ig targets CD68 in WB, IHC, IF/ICC, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Lysosomal contents of lung macrophages

• Full Name: CD68 molecule
• Calculated Molecular Weight: 37 kDa
• GenBank Accession Number: BC015557
• Gene Symbol: CD68
• Gene ID (NCBI): 968
• RRID: AB_2918458
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P34810
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	IF	Int Immunopharmacol; Diosgenin alters LPS-induced macrophage polarization by activating PPARγ/NF-κB signaling pathway; Authors - Xingchen Xiang
	IF	Phytomedicine; Koumine regulates macrophage M1/M2 polarization via TSPO, alleviating sepsis-associated liver injury in mice; Authors - Gui-Lin Jin
		Int Immunopharmacol; Macrophages regulates the transition of pericyte to peritoneal fibrosis through the GSDMD/IL-1β axis; Authors - Qiuyuan Shao
human	IF	Hepatology; Hepatocyte-derived MASP1-enriched small extracellular vesicles activate HSCs to promote liver fibrosis; Authors - Xianzhi Liu
	IF	Exp Neurol; Microglia trigger the structural plasticity of GABAergic neurons in the hippocampal CA1 region of a lipopolysaccharide-induced neuroinflammation model; Authors - Juan Chen
	WB,IHC	Int Immunopharmacol; Inhibition of aquaporin-9 ameliorates severe acute pancreatitis and associated lung injury by NLRP3 and Nrf2/HO-1 pathways; Authors - Jiawei Chen