CD74 Monoclonal antibody

CD74 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2D8A1

Cat no : 66390-1-Ig

Synonyms

CD74, DHLAG, HLADG, Ia GAMMA, Ii, p33

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Validation Data Gallery

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  WB analysis using 66390-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66390-1-Ig (CD74 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Ramos using 66390-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66390-1-Ig (CD74 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 66390-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66390-1-Ig (CD74 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of Raji using 66390-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD74 antibody (66390-1-Ig, Clone: 2D8A1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: Raji cells, Ramos cells, Daudi cells
• Positive IF detected in: Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF): IF : 1:400-1:1600

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

66390-1-Ig targets CD74 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD74 fusion protein Ag25503
• Full Name: CD74 molecule, major histocompatibility complex, class II invariant chain
• Calculated molecular weight: 34 kDa
• Observed molecular weight: 33 kDa
• GenBank accession number: BC018726
• Gene symbol: CD74
• Gene ID (NCBI): 972
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	WB	Int Immunol; Helicobacter urease suppresses cytotoxic CD8 + T cell responses through activating Myh9-dependent induction of PD-L1.; Authors - Jian Wu
	IF	Neural Regen Res; Single-nuclei RNA sequencing uncovers heterogenous transcriptional signatures in Parkinson's disease associated with nuclear receptor-related factor 1 defect; Authors - Piniel Alphayo Kambey