CD74 Monoclonal antibody

CD74 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

2D8A1

Cat no : 66390-1-Ig

Synonyms

CD74, DHLAG, HLADG, Ia GAMMA, Ii, p33

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Validation Data Gallery

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  WB analysis using 66390-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66390-1-Ig (CD74 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Raji cells, Ramos cells, Daudi cells

Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below

Product Information

66390-1-Ig targets CD74 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD74 fusion protein Ag25503
• Full Name: CD74 molecule, major histocompatibility complex, class II invariant chain
• Calculated molecular weight: 34 kDa
• Observed molecular weight: 33 kDa
• GenBank accession number: BC018726
• Gene symbol: CD74
• Gene ID (NCBI): 972
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
mouse	WB	Int Immunol; Helicobacter urease suppresses cytotoxic CD8 + T cell responses through activating Myh9-dependent induction of PD-L1.; Authors - Jian Wu