Anti-Human CD86 (BU63)

CD86 Monoclonal Antibody for FC, IF

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

IF, FC

Conjugate

Unconjugated

CloneNo.

BU63

Cat no : 65165-1-Ig

Synonyms

Activation B7 2 antigen, B7 2, B70, BU63, CD28LG2, CD86, CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, LAB72



Tested Applications

Positive IF detected inRaji cells
Positive FC detected inDaudi cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:500-1:2000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

65165-1-Ig targets CD86 in IF, FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen B-lymphoblastoid cell line ARH 77
Full Name CD86 molecule
Calculated Molecular Weight 329 aa, 38 kDa
GenBank Accession NumberBC040261
Gene Symbol CD86
Gene ID (NCBI) 942
RRIDAB_2881481
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

Protocols

Product Specific Protocols
IF protocol for CD86 antibody 65165-1-IgDownload protocol
FC protocol for CD86 antibody 65165-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols