APC Anti-Human CD86 (BU63)

• 

  FC experiment of human PBMCs using APC-65165

  1X10^6 human PBMCs were surface co-stained with FITC Anti-Human CD14 and 5 ul APC Anti-Human CD86 (APC-65165, Clone:BU63) or unstained. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using APC-65165

  1X10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD86 (APC-65165, Clone:BU63) or unstained. Cells were not fixed. Monocytes were gated.

CD86 Monoclonal Antibody for FC

Cat No. APC-65165

Clone No.BU63

Publications(5)

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

IF, FC

Synonyms

CD28LG2, BU63, B70, B7 2, Activation B7-2 antigen

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below
• FC: See 4 publications below

Product Information

APC-65165 targets CD86 in IF, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: B-lymphoblastoid cell line ARH 77
• Full Name: CD86 molecule
• Calculated Molecular Weight: 329 aa, 38 kDa
• GenBank Accession Number: BC040261
• Gene Symbol: CD86
• Gene ID (NCBI): 942
• ENSEMBL Gene ID: ENSG00000114013
• RRID: AB_2883037
• Conjugate: APC Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 650 nm / 660 nm
• Form: Liquid
• Purification Method: The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• UNIPROT ID: P42081
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

Publications

Species	Application	Title
human	FC	Cell Death Discov; A positive feedback loop: RAD18-YAP-TGF-β between triple-negative breast cancer and macrophages regulates cancer stemness and progression.; Authors - Xueqi Yan
human	FC	Inflammation; ROS-Induced Gingival Fibroblast Senescence: Implications in Exacerbating Inflammatory Responses in Periodontal Disease; Authors - Shuling Guo
human	FC	Front Immunol; Identification of a distinct cluster of GDF15high macrophages induced by in vitro differentiation exhibiting anti-inflammatory activities; Authors - Chaochao Dai
human	FC	J Agric Food Chem; Novel Collagen Analogs with Multicopy Mucin-Type Sequences for Multifunctional Enhancement Properties Using SUMO Fusion Tags; Authors - Zilong Zhao
human	IF	Adv Sci (Weinh); Senescent Fibroblasts Drive FAP/OLN Imbalance Through mTOR Signaling to Exacerbate Inflammation and Bone Resorption in Periodontitis; Authors - Chenghu Yin