|Positive FC detected in||human peripheral blood monocytes, Daudi cells|
|This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.|
|Sample-dependent, check data in validation data gallery|
APC-65165 targets CD86 in FC applications and shows reactivity with Human samples.
|Host / Isotype||Mouse / Mouse IgG1|
|Immunogen||B-lymphoblastoid cell line ARH 77|
|Full Name||CD86 molecule|
|Calculated molecular weight||329 aa, 38 kDa|
|GenBank accession number||BC040261|
|Gene ID (NCBI)||942|
|Conjugate||APC Fluorescent Dye|
|Excitation/Emission maxima wavelengths||650 nm / 660 nm|
|Purification Method||The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.|
|Storage Buffer||PBS with 0.1% sodium azide.|
|Storage Conditions||Store at 2-8°C. Avoid exposure to light.|
CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.