APC Anti-Human CD86 (BU63)

CD86 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG1

Reactivity

Human

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

BU63

Cat no : APC-65165

Synonyms

Activation B7 2 antigen, B7 2, B70, BU63, CD28LG2, CD86, CD86 molecule, CTLA 4 counter receptor B7.2, FUN 1, LAB72



Tested Applications

Positive FC detected inhuman peripheral blood monocytes, Daudi cells

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Product Information

APC-65165 targets CD86 in FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / Mouse IgG1
Class Monoclonal
Type Antibody
Immunogen B-lymphoblastoid cell line ARH 77
Full Name CD86 molecule
Calculated molecular weight 329 aa, 38 kDa
GenBank accession numberBC040261
Gene symbol CD86
Gene ID (NCBI) 942
Conjugate APC Fluorescent Dye
Excitation/Emission maxima wavelengths650 nm/660 nm
Form Liquid
Purification Method The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.

Protocols

Product Specific Protocols
FC protocol for APC CD86 antibody APC-65165Download protocol
Standard Protocols
Click here to view our Standard Protocols