CoraLite® Plus 488 Anti-Mouse CD86 (GL1)

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  FC experiment of C57BL/6 mouse splenocytes using CL488-65068

  1X10^6 unstimulated or LPS stimulated (5 ug/mL, 3 days) C57BL/6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD86 (CL488-65068, Clone: GL1). Cells were not fixed.

CD86 Monoclonal Antibody for FC

Cat No. CL488-65068

Clone No.GL1

Publications(1)

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

FC

Synonyms

Cd28l2, B7-2, B70, B7.2, B7 2

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Tested Applications

• Positive FC detected in: LPS treated C57BL/6 mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 1 publications below

Product Information

CL488-65068 targets CD86 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: LPS-activated CBA/Ca mouse spleen B cells
• Full Name: CD86 antigen
• GenBank Accession Number: BC013807
• Gene Symbol: Cd86
• Gene ID (NCBI): 12524
• RRID: AB_2883160
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P42082
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

Publications

Species	Application	Title
mouse	FC	Respir Res; YTHDF1-mediated m6A modification of GBP4 promotes M1 macrophage polarization in acute lung injury; Authors - Fengan Cao