Tested Applications
Positive FC detected in | LPS treated C57BL/6 mouse splenocytes |
Recommended dilution
Application | Dilution |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
FC | See 1 publications below |
Product Information
CL488-65068 targets CD86 in FC applications and shows reactivity with mouse samples.
Tested Reactivity | mouse |
Cited Reactivity | mouse |
Host / Isotype | Rat / IgG2a, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | LPS-activated CBA/Ca mouse spleen B cells Predict reactive species |
Full Name | CD86 antigen |
GenBank Accession Number | BC013807 |
Gene Symbol | Cd86 |
Gene ID (NCBI) | 12524 |
RRID | AB_2883160 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Affinity purification |
UNIPROT ID | P42082 |
Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 488 CD86 antibody CL488-65068 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Respir Res YTHDF1-mediated m6A modification of GBP4 promotes M1 macrophage polarization in acute lung injury |