CoraLite® Plus 488 Anti-Mouse CD86 (GL1)

CD86 Monoclonal Antibody for FC
Cat No. CL488-65068
Clone No.GL1

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse

Applications

FC

Cd28l2, B7-2, B70, B7.2, B7 2

Formulation:  PBS and Azide
PBS and Azide
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive FC detected inLPS treated C57BL/6 mouse splenocytes

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

FCSee 1 publications below

Product Information

CL488-65068 targets CD86 in FC applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Cited Reactivitymouse
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen LPS-activated CBA/Ca mouse spleen B cells Predict reactive species
Full Name CD86 antigen
GenBank Accession NumberBC013807
Gene Symbol Cd86
Gene ID (NCBI) 12524
RRIDAB_2883160
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP42082
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD86 (also known as B7-2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. (PMID: 7513726; 1847722; 11029388; 27591335)

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 CD86 antibody CL488-65068Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseFC

Respir Res

YTHDF1-mediated m6A modification of GBP4 promotes M1 macrophage polarization in acute lung injury

Authors - Fengan Cao
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