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  • Western Blot (WB) analysis of various lysates using CD86 (C-terminal) Polyclonal antibody (26903-1-AP)

    WB analysis using 26903-1-AP (same clone as 26903-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26903-1-AP (CD86 (C-terminal) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26903-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD86 (C-terminal) Polyclonal antibody (26903-1-AP)

    IHC staining of human tonsillitis using 26903-1-AP (same clone as 26903-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26903-1-AP (CD86 (C-terminal) antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26903-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD86 (C-terminal) Polyclonal antibody (26903-1-AP)

    IHC staining of human tonsillitis using 26903-1-AP (same clone as 26903-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26903-1-AP (CD86 (C-terminal) antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26903-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CD86 (C-terminal) Polyclonal antibody (26903-1-AP)

    IF Staining of mouse brain using 26903-1-AP (same clone as 26903-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using CD86 (C-terminal) antibody (26903-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 26903-1-PBS in a different storage buffer formulation.

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Product Information

26903-1-PBS targets CD86 (C-terminal) in WB, IHC, IF-Fro, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD86 (C-terminal) fusion protein Ag25432 Predict reactive species
Full Name CD86 molecule
Calculated Molecular Weight 329 aa, 38 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC040261
Gene Symbol CD86
Gene ID (NCBI) 942
ENSEMBL Gene IDENSG00000114013
RRIDAB_2880677
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42081
Storage Buffer PBS only, pH 7.3.
Storage ConditionsStore at -80°C.

Background Information

CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response.