CoraLite® Plus 488 Anti-Human CD8a (OKT8)

CD8a Monoclonal Antibody for FC, IF

Host / Isotype

Mouse / IgG2a, kappa

Reactivity

Human

Applications

IF, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

OKT8

Cat no : CL488-65135

Synonyms

CD8, CD8A, CD8a molecule, Leu2, MAL, p32



Tested Applications

Positive IF detected inhuman peripheral blood mononuclear cells
Positive FC detected inhuman peripheral blood lymphocytes

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65135 targets CD8a in IF, FC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a, kappa
Class Monoclonal
Type Antibody
Immunogen Human thymocytes
Full Name CD8a molecule
Calculated Molecular Weight 235 aa, 26 kDa
GenBank Accession NumberBC025715
Gene Symbol CD8A
Gene ID (NCBI) 925
RRIDAB_2883197
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells , on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD8a antibody CL488-65135Download protocol
FC protocol for CL Plus 488 CD8a antibody CL488-65135Download protocol
Standard Protocols
Click here to view our Standard Protocols