Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
APC-FcA65506 targets CD8a in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Human thymocytes Predict reactive species |
| Full Name | CD8a molecule |
| Calculated Molecular Weight | 235 aa, 26 kDa |
| GenBank Accession Number | BC025715 |
| Gene Symbol | CD8A |
| Gene ID (NCBI) | 925 |
| ENSEMBL Gene ID | ENSG00000153563 |
| RRID | AB_3746408 |
| Conjugate | APC Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 650 nm / 660 nm |
| Excitation Laser | Red Laser (633 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P01732 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD8 is a transmembrane glycoprotein composed of two disulfide-linked chains. It can be present as a homodimer of CD8α or as a heterodimer of CD8α and CD8β (PMID: 3264320; 8253791). CD8 is found on most thymocytes. The majority of class I-restricted T cells express mostly the CD8αβ heterodimer while CD8αα homodimers alone have been found on some gut intraepithelial T cells, on some T cell receptor (TCR) γδ T cells and on NK cells (PMID: 2111591; 1831127; 8420975). CD8 acts as a co-receptor that binds to MHC class-I and participates in cytotoxic T-cell activation (PMID: 8499079). During T cell development, CD8 is required for positive selection of CD4-/CD8+ T cells (PMID: 1968084).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for APC CD8a antibody APC-FcA65506 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
One reviewer (Eduardo Canotilho, July 2025) gave this APC-conjugated anti-CD8 antibody a 5-star rating after testing it by flow cytometry on human PBMCs. He reported excellent titration performance, noting that signal intensity increased proportionally with concentration and that clear separation between positive and negative populations was visible even at the lowest tested dilution (0.25:150). Optimal staining was achieved at 2:150 and 4:150, with the 4:150 dilution providing improved resolution between CD8^dim and CD8^bright populations due to enhanced signal separation.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Eduardo (Verified Customer) (07-02-2025) | The titration results for the anti-CD8 antibody conjugated to APC show excellent performance across a range of concentrations. Notably, the signal intensity increases proportionally with antibody concentration, with clear separation between positive and negative populations beginning as early as the 0.25:150 dilution. Optimal staining appears to be achieved at the 2:150 and 4:150 dilutions, with 4:150 offering improved resolution between CD8^dim and CD8^bright populations due to enhanced signal separation.
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