CENPE Polyclonal antibody

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• CENPE Polyclonal antibody (28142-1-AP)

• 

  WB analysis using 28142-1-AP

  HEK-293 cells lysate were subjected to SDS PAGE followed by western blot with 28142-1-AP (CENPE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 28142-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 28142-1-AP (CENPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 28142-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 28142-1-AP (CENPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 28142-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 28142-1-AP (CENPE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

CENPE Polyclonal Antibody for WB, ELISA

Cat No. 28142-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

Kinesin 7, CENP E, Centromere associated protein E, Centromere protein E, Kinesin related protein CENPE

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 5 publications below

Product Information

28142-1-AP targets CENPE in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27230

  Product name: Recombinant human CENPE protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 2401-2500 aa of NM_001286734

  Sequence: MESKIIKMQKELEVTNDIIAKLQAKVHESNKCLEKTKETIQVLQDKVALGAKPYKEEIEDLKMKLVKIDLEKMKNAKEFEKEISATKATVEYQKEVIRLLR

• Full Name: centromere protein E, 312kDa
• Calculated Molecular Weight: 316 kDa
• Observed Molecular Weight: 300-316 kDa
• GenBank Accession Number: NM_001286734
• Gene Symbol: CENPE
• Gene ID (NCBI): 1062
• RRID: AB_2881072
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q02224
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Centromeric protein E (CENPE) is also named Kinesin-7. CENPE, a member of the centromere protein (CENP) family, functions as a microtubule plus-end directed motor protein that is upregulated during the G2 phase (PMID: 31115500, PMID: 7851898, PMID: 38560520). It plays a crucial role in the spindle assembly checkpoint (SAC), chromosome alignment, and microtubule capture at kinetochores during mitosis (PMID: 30784092). CENPE is essential for mitotic white matter, which accumulates during the G2 phase of the cell cycle, is consumed throughout mitosis and degrades later in the cell cycle (PMID: 33862458). CENPE regulates the cell cycle by targeting the transcription of CDK1 in the nucleus (PMID: 33862458). Studies on tumor cells have shown that CENPE plays an important role in the regulation of cell proliferation, apoptosis, migration and other activities (PMID: 33862458).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Biochim Biophys Acta Mol Cell Res; Kinesin-7 CENP-E is essential for chromosome alignment and spindle assembly of mouse spermatocytes.; Authors - Zhen-Yu She
human	WB	Am J Transl Res; Kinesin family members KIF2C/4A/10/11/14/18B/20A/23 predict poor prognosis and promote cell proliferation in hepatocellular carcinoma.; Authors - Xishan Li
human	WB	Curr Res Transl Med; Identifying potential prognosis markers in relapsed multiple myeloma via integrated bioinformatics analysis and biological experiments; Authors - Yong Xu
human	IHC	Cancer Cell Int; Characterization of G2/M checkpoint classifier for personalized treatment in uterine corpus endometrial carcinoma; Authors - Yiming Liu
human	WB	J Cancer; Development and Validation of the novel Cuproptosis- and Immune-related Signature for Predicting Prognosis in Hepatocellular Carcinoma; Authors - Yongping Zhang
mouse,human	IHC	IET Syst Biol; Mechanistic Investigation of Nitidine Chloride-Mediated Anti-Colorectal Cancer Activity: Centromere-Associated Protein E Targeting via Integrated Molecular Dynamics, Spatial Transcriptomic and Single-Cell Approaches.; Authors - Bin Li