CENPF Recombinant monoclonal antibody, PBS Only

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  IF Staining of HeLa using 84865-4-RR (same clone as 84865-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CENPF antibody (84865-4-RR, Clone: 242277G9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84865-4-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84865-4-RR (same clone as 84865-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CENPF antibody (84865-4-RR, Clone: 242277G9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84865-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84865-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84865-4-RR against Human CENPF were performed. The affinity constant is 11.1 nM.

CENPF Uni-rAb^® Recombinant Antibody for IF/ICC, Indirect ELISA

Cat No. 84865-4-PBS

Clone No.242277G9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Synonyms

242277G9, CENF, CENP-F, PRO1779

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Product Information

84865-4-PBS targets CENPF in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag29665

  Product name: Recombinant human CENPF protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 3011-3210 aa of NM_016343

  Sequence: SVTEKRLSSGQNKASGKRQRSSGIWENGRGPTPATPESFSKKSKKAVMSGIHPAEDTEGTEFEPEGLPEVVKKGFADIPTGKTSPYILRRTTMATRTSPRLAAQKLALSPLSLGKENLAESSKPTAGGSRSQKVKVAQRSPVDSGTILREPTTKSVPVNNLPERSPTDSPREGLRVKRGRLVPSPKAGLESNGSENCKVQ

• Full Name: centromere protein F, 350/400ka (mitosin)
• Calculated Molecular Weight: 368 kDa
• GenBank Accession Number: NM_016343
• Gene Symbol: CENPF
• Gene ID (NCBI): 1063
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P49454
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).