CEP164 Polyclonal antibody

CEP164 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, Canine and More (1)





Cat no : 22227-1-AP


centrosomal protein 164kDa, Centrosomal protein of 164 kDa, CEP164, KIAA1052

Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inRPE1 cells
Positive IHC detected inhuman colon tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, A549 cells, PC-3 cells, MDCK cells, hTERT-RPE1 cells

Recommended dilution

Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22227-1-AP targets CEP164 in WB,IP,IHC,IF,ELISA applications and shows reactivity with human, Canine samples.

Tested Reactivity human, Canine
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEP164 fusion protein Ag17570
Full Name centrosomal protein 164kDa
Calculated Molecular Weight 1460 aa, 164 kDa
Observed Molecular Weight 164 kDa
GenBank Accession NumberBC000602
Gene Symbol CEP164
Gene ID (NCBI) 22897
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP164, also named as KIAA1052 or NPHP15, is a 1460 amino acid protein, which contains 1 WW domain. CEP164 localizes in the microtubule organizing center and is expressed in several cell lines. CEP164 plays a role in microtubule organization and/or maintenance for the formation of primary cilia, a microtubule-based structure that protrudes from the surface of epithelial cells. CEP164 plays a critical role in G2/M checkpoint and nuclear divisions. The expression of CEP164 is normally limited to the mother centriole, and CEP164 can be used as a useful marker for mother centriole.


Product Specific Protocols
WB protocol for CEP164 antibody 22227-1-APDownload protocol
IHC protocol for CEP164 antibody 22227-1-APDownload protocol
IF protocol for CEP164 antibody 22227-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Res

NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.

Authors - Min Liu

Nat Commun

M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole.

Authors - Ning Huang

Nat Commun

Genetic link between renal birth defects and congenital heart disease.

Authors - Jovenal T San Agustin

Nat Commun

DNA replication licensing factor Cdc6 and Plk4 kinase antagonistically regulate centrosome duplication via Sas-6.

Authors - Xiaowei Xu


ANKRD26 recruits PIDD1 to centriolar distal appendages to activate the PIDDosome following centrosome amplification.

Authors - Lauren T Evans

Curr Biol

Rab34 is necessary for early stages of intracellular ciliogenesis.

Authors - Michael W Stuck
  • KO Validated


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Ludovic (Verified Customer) (02-27-2024)

A fabulous antibody to identify the distal appendage of the primary cilium. Never seen an antibody that works so well!

  • Applications: Immunofluorescence, Cell culture
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human cell line

Elisa (Verified Customer) (04-04-2023)

H9 cells stained for Hoechst (DNA marker, in green), Cep164 (mother centriole distal appendage marker, in green) and g-Tubulin (pericentriolar matrix marker, in magenta). H9 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:300 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: H9 cells
CEP164 Antibody Immunofluorescence validation (1:300 dilution) in H9 cells (Cat no:22227-1-AP)

Pierrick (Verified Customer) (10-24-2019)

Great antibody for immunofluorescence on human cells.Works well in WB

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: CEP164 IF 1/1000 to 1/2000 and WB 1/1000
  • Cell Tissue Type: RPE1, HEK290, HeLa Kyoto