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CEPT1 Polyclonal antibody

CEPT1 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 20496-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

1-alkenyl-2-acylglycerol choline phosphotransferase, choline/ethanolamine phosphotransferase 1, EC:2.7.8.1, EC:2.7.8.22, hCEPT1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive IHC detected inmouse liver tissue, human cervical cancer tissue, human pancreas tissue, human tonsillitis tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:20-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 4 publications below

Product Information

20496-1-AP targets CEPT1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14140

Product name: Recombinant human CEPT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-91 aa of BC032610

Sequence: MSGHRSTRKRCGDSHPESPVGFGHMSTTGCVLNKLFQLPTPPLSRHQLKRLEEHRYQSAGRSLLEPLMQGYWEWLVRRVPSWIAPNLITII

Predict reactive species
Full Name choline/ethanolamine phosphotransferase 1
Calculated Molecular Weight 416 aa, 47 kDa
GenBank Accession NumberBC032610
Gene Symbol CEPT1
Gene ID (NCBI) 10390
RRIDAB_10694283
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6K0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Choline-enthanolamine phosphotransferase 1 (CEPT1) is a 47-kDa membrane-bound protein that catalyzes the terminal step of the Kennedy pathway. CEPT1 is encoded by the Cept1 gene and is responsible for phosphatidylcholine (PC) and phosphatidylethanolamine (PE) production (PMID: 33214136, 30030520).

Protocols

Product Specific Protocols
IHC protocol for CEPT1 antibody 20496-1-APDownload protocol
IF protocol for CEPT1 antibody 20496-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Protein Cell

Proteomic analysis of ferroptosis pathways reveals a role of CEPT1 in suppressing ferroptosis

Authors - Xiaoguang Liu
human

Cell Res

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Authors - Pengli Zheng
humanIF

J Lipid Res

Diabetes Adversely Affects Phospholipid Profiles in Human Carotid Artery Endarterectomy Plaques.

Authors - Mohamed A Zayed
humanIF

Hepatol Commun

Dysregulation of lipid metabolism in the pseudolobule promotes region-specific autophagy in hepatitis B liver cirrhosis

Authors - Wenjun Pu
humanIF

J Hazard Mater

YTHDC2 mediated RNA m6A modification contributes to PM2.5-induced hepatic steatosis

Authors - Zhipeng Yan
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