Complement factor H Polyclonal antibody

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  WB analysis using 12748-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 12748-1-AP (Complement factor H antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human blood using 12748-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 12748-1-AP (Complement factor H antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 12748-1-AP

  IP result of anti-Complement factor H (IP:12748-1-AP, 4ug; Detection:12748-1-AP 1:1000) with human plasma lysate 4000ug.

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  IHC staining of human liver cancer using 12748-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12748-1-AP (Complement factor H antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12748-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12748-1-AP (Complement factor H antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human normal colon using 12748-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12748-1-AP (Complement factor H antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human normal colon using 12748-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12748-1-AP (Complement factor H antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human liver cancer using 12748-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Complement factor H antibody (12748-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12748-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Complement factor H antibody (12748-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Complement factor H Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 12748-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

CFH, FHL1, FH, Factor H, ARMS1

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Tested Applications

• Positive WB detected in: human plasma, human blood
• Positive IP detected in: human plasma tissue
• Positive IHC detected in: human liver cancer tissue, human normal colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human liver cancer tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12748-1-AP targets Complement factor H in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Complement factor H fusion protein Ag3482
• Full Name: complement factor H
• Calculated Molecular Weight: 1231 aa, 139 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC037285
• Gene Symbol: Complement factor H
• Gene ID (NCBI): 3075
• RRID: AB_10642946
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08603
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Complement factor H (CFH) is an abundant plasma glycoprotein that regulates the function of the alternative complement pathway in the fluid phase and on cellular surfaces (PMID: 2963625). Factor H binds to C3b, accelerates the decay of the alternative pathway C3-convertase (C3bBb), and acts as a cofactor for the factor I-mediated proteolytic inactivation of C3b (PMID: 15163532). It plays a critical role in the homeostasis of the complement system in plasma and in the protection of bystander host cells and tissues from damage by complement activation. Abnormalities in factor H have been associated with renal disease (PMID: 18190458).

Publications

Species	Application	Title
human	WB	Immunol Res; The influences of α-hemolytic Streptococcus on class switching and complement activation of human tonsillar cells in IgA nephropathy.; Authors - Muyao Ye