Validation Data Gallery
|Positive WB detected in||Jurkat cells, HepG2 cells, HeLa cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||mouse skeletal muscle tissue, human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10394-1-AP targets CFLAR/FLIP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CFLAR/FLIP fusion protein Ag0593|
|Full Name||CASP8 and FADD-like apoptosis regulator|
|Calculated molecular weight||52 kDa|
|Observed molecular weight||20-35 kDa, 50-55 kDa|
|GenBank accession number||BC001602|
|Gene ID (NCBI)||8837|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CFLAR, also named as CASH, CASP8AP1, CLARP, MRIT, Casper, c-FLIP and I-FLICE, belongs to the peptidase C14A family. It is an apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. CFLAR acts as an inhibitor of TNFRSF6 mediated apoptosis. It can be cleaved to be 2 fragments P43 and P12. P43 is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. CFLAR lacks enzymatic (caspase) activity.
|Product Specific Protocols|
|WB protocol for CFLAR/FLIP antibody 10394-1-AP||Download protocol|
|IHC protocol for CFLAR/FLIP antibody 10394-1-AP||Download protocol|
|IF protocol for CFLAR/FLIP antibody 10394-1-AP||Download protocol|
|IP protocol for CFLAR/FLIP antibody 10394-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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