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CFLAR/FLIP Polyclonal antibody

CFLAR/FLIP Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 10394-1-AP


c FLIP, c FLIPL, c FLIPR, c FLIPS, CASH, CASP8AP1, Caspase eight related protein, Caspase homolog, Casper, CFLAR, CFLAR/FLIP, CLARP, FLAME, FLAME 1, FLAME1, FLIP, I FLICE, Inhibitor of FLICE, MRIT, Usurpin

Tested Applications

Positive WB detected inJurkat cells, HepG2 cells, HeLa cells
Positive IP detected inJurkat cells
Positive IHC detected inmouse skeletal muscle tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10394-1-AP targets CFLAR/FLIP in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CFLAR/FLIP fusion protein Ag0593
Full Name CASP8 and FADD-like apoptosis regulator
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 20-35 kDa, 50-55 kDa
GenBank Accession NumberBC001602
Gene Symbol CFLAR
Gene ID (NCBI) 8837
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CFLAR, also named CASH, CASP8AP1, CLARP, MRIT, Casper, c-FLIP, and I-FLICE, belongs to the peptidase C14A family. It is an apoptosis regulator protein that may function as a crucial link between cell survival and cell death pathways in mammalian cells. CFLAR acts as an inhibitor of TNFRSF6-mediated apoptosis. It can be cleaved to be 2 fragments P43 and P12. P43 is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full-length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. CFLAR lacks enzymatic (caspase) activity.


Product Specific Protocols
WB protocol for CFLAR/FLIP antibody 10394-1-APDownload protocol
IHC protocol for CFLAR/FLIP antibody 10394-1-APDownload protocol
IF protocol for CFLAR/FLIP antibody 10394-1-APDownload protocol
IP protocol for CFLAR/FLIP antibody 10394-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cancer Res

DAB2IP is a bifunctional tumor suppressor that regulates wildtype RAS and inflammatory cascades in KRAS mutant colon cancer

Authors - Abigail L Miller

Cell Death Differ

Coordinated repression of BIM and PUMA by Epstein-Barr virus latent genes maintains the survival of Burkitt lymphoma cells.

Authors - Leah Fitzsimmons

J Cell Mol Med

Exercise ameliorates insulin resistance and improves ASK1-mediated insulin signalling in obese rats.

Authors - Yong Zhang

Biomed Pharmacother

Regorafenib suppresses epidermal growth factor receptor signaling-modulated progression of colorectal cancer.

Authors - Yu-Chang Liu

Oncol Lett

YM155 sensitizes HeLa cells to TRAIL-mediated apoptosis via cFLIP and survivin downregulation.

Authors - Arun Pandian Chandrasekaran

Mol Med Rep

Chidamide induces necroptosis via regulation of c‑FLIPL expression in Jurkat and HUT‑78 cells.

Authors - Zuofei Chi