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  • KD/KO Validated

CFLAR/FLIP Polyclonal antibody

CFLAR/FLIP Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10394-1-AP

Synonyms

c FLIP, c FLIPL, c FLIPR, c FLIPS, CASH, CASP8AP1, Caspase eight related protein, Caspase homolog, Casper, CFLAR, CLARP, FLAME, FLAME 1, FLAME1, FLIP, I FLICE, Inhibitor of FLICE, MRIT, Usurpin



Tested Applications

Positive WB detected inJurkat cells, HepG2 cells, HeLa cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

10394-1-AP targets CFLAR/FLIP in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CFLAR/FLIP fusion protein Ag0593
Full Name CASP8 and FADD-like apoptosis regulator
Calculated molecular weight 52 kDa
Observed molecular weight 30-35 kDa, 50-55 kDa
GenBank accession numberBC001602
Gene symbol CFLAR
Gene ID (NCBI) 8837
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CFLAR, also named as CASH, CASP8AP1, CLARP, MRIT, Casper, c-FLIP and I-FLICE, belongs to the peptidase C14A family. It is an apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. CFLAR acts as an inhibitor of TNFRSF6 mediated apoptosis. It can be cleaved to be 2 fragments P43 and P12. P43 is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. CFLAR lacks enzymatic (caspase) activity.

Protocols

Product Specific Protocols
WB protocol for CFLAR/FLIP antibody 10394-1-APDownload protocol
IHC protocol for CFLAR/FLIP antibody 10394-1-APDownload protocol
IF protocol for CFLAR/FLIP antibody 10394-1-APDownload protocol
IP protocol for CFLAR/FLIP antibody 10394-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Biochem Biophys Res Commun

CRISPR-mediated upregulation of DR5 and downregulation of cFLIP synergistically sensitize HeLa cells to TRAIL-mediated apoptosis.

Authors - Naresh Poondla
humanWB

Cell Death Dis

Cellular FLICE-like inhibitory protein (cFLIP) critically maintains apoptotic resistance in human lens epithelial cells.

Authors - Jingru Huangfu
  • KD Validated
humanWB

Thorac Cancer

PMID: 26273359

Authors - Xuan Zhang
humanWB

Mol Med Rep

Chidamide induces necroptosis via regulation of c‑FLIPL expression in Jurkat and HUT‑78 cells.

Authors - Zuofei Chi
humanWB

Oncol Lett

YM155 sensitizes HeLa cells to TRAIL-mediated apoptosis via cFLIP and survivin downregulation.

Authors - Arun Pandian Chandrasekaran
humanWB,IHC

Biomed Pharmacother

Regorafenib suppresses epidermal growth factor receptor signaling-modulated progression of colorectal cancer.

Authors - Yu-Chang Liu