|Positive WB detected in||Jurkat cells, HepG2 cells, HeLa cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10394-1-AP targets CFLAR/FLIP in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||CFLAR/FLIP fusion protein Ag0593|
|Full Name||CASP8 and FADD-like apoptosis regulator|
|Calculated molecular weight||52 kDa|
|Observed molecular weight||30-35 kDa, 50-55 kDa|
|GenBank accession number||BC001602|
|Gene ID (NCBI)||8837|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CFLAR, also named as CASH, CASP8AP1, CLARP, MRIT, Casper, c-FLIP and I-FLICE, belongs to the peptidase C14A family. It is an apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. CFLAR acts as an inhibitor of TNFRSF6 mediated apoptosis. It can be cleaved to be 2 fragments P43 and P12. P43 is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. CFLAR lacks enzymatic (caspase) activity.
|Product Specific Protocols|
|WB protocol for CFLAR/FLIP antibody 10394-1-AP||Download protocol|
|IHC protocol for CFLAR/FLIP antibody 10394-1-AP||Download protocol|
|IF protocol for CFLAR/FLIP antibody 10394-1-AP||Download protocol|
|IP protocol for CFLAR/FLIP antibody 10394-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Biochem Biophys Res Commun
CRISPR-mediated upregulation of DR5 and downregulation of cFLIP synergistically sensitize HeLa cells to TRAIL-mediated apoptosis.
Cell Death Dis
Cellular FLICE-like inhibitory protein (cFLIP) critically maintains apoptotic resistance in human lens epithelial cells.
Mol Med Rep
Chidamide induces necroptosis via regulation of c‑FLIPL expression in Jurkat and HUT‑78 cells.
YM155 sensitizes HeLa cells to TRAIL-mediated apoptosis via cFLIP and survivin downregulation.
Regorafenib suppresses epidermal growth factor receptor signaling-modulated progression of colorectal cancer.