CFLAR/FLIP Polyclonal antibody, PBS Only

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  WB analysis of Jurkat using 10394-1-AP (same clone as 10394-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 10394-1-AP (same clone as 10394-1-PBS)

  WB result of CFLAR/FLIP antibody (10394-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CFLAR/FLIP transfected HepG2 cells. This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10394-1-AP (same clone as 10394-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 10394-1-AP (same clone as 10394-1-PBS)

  IP result of anti-CFLAR/FLIP (IP:10394-1-AP, 4ug; Detection:10394-1-AP 1:500) with Jurkat cells lysate 4000ug. This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

• 

  IP experiment of Jurkat using 10394-1-AP (same clone as 10394-1-PBS)

  IP result of anti-CFLAR/FLIP (IP:10394-1-AP, 4ug; Detection:10394-1-AP 1:500) with Jurkat cells lysate 4000ug. This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 10394-1-AP (same clone as 10394-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 10394-1-AP (CFLAR/FLIP antibody at dilution of 1:50. This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 10394-1-AP (same clone as 10394-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 10394-1-AP (same clone as 10394-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10394-1-AP (same clone as 10394-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CFLAR/FLIP antibody (10394-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

CFLAR/FLIP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10394-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CFLAR, c FLIP, c FLIPL, c FLIPR, c FLIPS

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Product Information

10394-1-PBS targets CFLAR/FLIP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CFLAR/FLIP fusion protein Ag0593
• Full Name: CASP8 and FADD-like apoptosis regulator
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 20-35 kDa, 50-55 kDa
• GenBank Accession Number: BC001602
• Gene Symbol: FLIP
• Gene ID (NCBI): 8837
• RRID: AB_2081087
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15519
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CFLAR, also named as CASH, CASP8AP1, CLARP, MRIT, Casper, c-FLIP and I-FLICE, belongs to the peptidase C14A family. It is an apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. CFLAR acts as an inhibitor of TNFRSF6 mediated apoptosis. It can be cleaved to be 2 fragments P43 and P12. P43 is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. CFLAR lacks enzymatic (caspase) activity.