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CH25H Polyclonal antibody

CH25H Polyclonal Antibody for IHC, IP,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 16734-1-AP

Synonyms

C25H, CH25H, cholesterol 25 hydroxylase, Cholesterol 25 monooxygenase, h25OH

Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of mouse liver tissue using CH25H Polyclonal antibody (16734-1-AP)

    IP experiment of mouse liver using 16734-1-AP

    IP result of anti-CH25H (IP:16734-1-AP, 4ug; Detection:16734-1-AP 1:300) with mouse liver tissue lysate 5200ug.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CH25H Polyclonal antibody (16734-1-AP)

    IHC staining of mouse liver using 16734-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16734-1-AP (CH25H Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using CH25H Polyclonal antibody (16734-1-AP)

    IHC staining of mouse liver using 16734-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16734-1-AP (CH25H Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IP detected inmouse liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16734-1-AP targets CH25H in IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CH25H fusion protein Ag9706
Full Name cholesterol 25-hydroxylase
Calculated Molecular Weight 272 aa, 32 kDa
GenBank Accession NumberBC017843
Gene Symbol CH25H
Gene ID (NCBI) 9023
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.