CHI3L1/YKL40 Polyclonal antibody

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  WB analysis of THP-1 using 12036-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 12036-1-AP (CHI3L1/YKL40 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 12036-1-AP

  WB result of CHI3L1/YKL40 antibody (12036-1-AP; 1:10000; room temperature for 1.5 hours) with wild-type and CHI3L1/YKL40 knockout THP-1 cells.

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  IHC staining of human liver using 12036-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12036-1-AP (CHI3L1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 12036-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12036-1-AP (CHI3L1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary tumor using 12036-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12036-1-AP (CHI3L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 12036-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12036-1-AP (CHI3L1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse brain using 12036-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CHI3L1/YKL40 antibody (12036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 12036-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CHI3L1/YKL40 antibody (12036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of THP-1 using 12036-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CHI3L1/YKL40 antibody (12036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

CHI3L1/YKL40 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 12036-1-AP

Publications(28)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

CHI3L1, CGP 39, CGP-39, Chitinase 3 like protein 1, Chitinase-3-like protein 1

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Tested Applications

• Positive WB detected in: THP-1 cells
• Positive IHC detected in: human liver tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IHC: See 14 publications below
• IF: See 10 publications below

Product Information

12036-1-AP targets CHI3L1/YKL40 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHI3L1/YKL40 fusion protein Ag2710
• Full Name: chitinase 3-like 1 (cartilage glycoprotein-39)
• Calculated Molecular Weight: 383 aa, 43 kDa
• Observed Molecular Weight: 40, 33 kDa
• GenBank Accession Number: BC008568
• Gene Symbol: CHI3L1
• Gene ID (NCBI): 1116
• RRID: AB_2877819
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P36222
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein. (PMID:21763261)

Publications

Species	Application	Title
mouse	IF	Sci Bull (Beijing); Single-cell profile reveals the landscape of cardiac immunity and identifies a cardio-protective Ym-1hi neutrophil in myocardial ischemia-reperfusion injury; Authors - Yalan Dong
human	WB	J Neurol Neurosurg Psychiatry; Molecules of senescent glial cells differentiate Alzheimer's disease from ageing; Authors - Linbin Dai
mouse	WB,IHC,IF	Elife; Chitinase 3-like-1 contributes to acetaminophen-induced liver injury by promoting hepatic platelet recruitment.; Authors - Zhao Shan
human	IHC	Genomics; MIN score predicts primary response to infliximab/adalimumab and vedolizumab therapy in patients with inflammatory bowel diseases.; Authors - Yuan Shi
human	IF	Glia; SSEA-4 and YKL-40 positive progenitor subtypes in the subventricular zone of developing human neocortex.; Authors - Christian B Brøchner
human	IHC	Front Oncol; BYSL Promotes Glioblastoma Cell Migration, Invasion, and Mesenchymal Transition Through the GSK-3β/β-Catenin Signaling Pathway.; Authors - Zhuang Sha