CHI3L1/YKL40 Polyclonal antibody

CHI3L1/YKL40 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 12036-1-AP

Synonyms

39 kDa synovial protein, ASRT7, Cartilage glycoprotein 39, CGP 39, CHI3L1, CHI3L1/YKL40, Chitinase 3 like protein 1, Chitinase 3-like 1, GP 39, GP39, HC gp39, hCGP 39, HCGP 3P, YKL 40, YKL40, YYL 40

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Validation Data Gallery

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  WB analysis of THP-1 using 12036-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 12036-1-AP (CHI3L1/YKL40 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 12036-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12036-1-AP (CHI3L1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 12036-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12036-1-AP (CHI3L1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary tumor using 12036-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12036-1-AP (CHI3L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 12036-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12036-1-AP (CHI3L1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: THP-1 cells
• Positive IHC detected in: human liver tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Product Information

12036-1-AP targets CHI3L1/YKL40 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHI3L1/YKL40 fusion protein Ag2710
• Full Name: chitinase 3-like 1 (cartilage glycoprotein-39)
• Calculated molecular weight: 383 aa, 43 kDa
• Observed molecular weight: 39 kDa
• GenBank accession number: BC008568
• Gene symbol: CHI3L1
• Gene ID (NCBI): 1116
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein. (PMID:21763261)

Publications

Species	Application	Title
human	WB	Cancer Manag Res; LINC00963 Promotes Ovarian Cancer Proliferation, Migration and EMT via the miR-378g /CHI3L1 Axis.; Authors - Wei Liu
rat	IHC,IF	Biochem Biophys Res Commun; Effects of chitinase-3-like protein 1 on brain death-induced hepatocyte apoptosis via PAR2-JNK-caspase-3.; Authors - Ze-Xin Li
mouse	IHC	Pharmaceutics; Chitooligosaccharides Improve the Efficacy of Checkpoint Inhibitors in a Mouse Model of Lung Cancer.; Authors - Astrid Zedlitz Johansen
human	IHC	Front Oncol; BYSL Promotes Glioblastoma Cell Migration, Invasion, and Mesenchymal Transition Through the GSK-3β/β-Catenin Signaling Pathway.; Authors - Zhuang Sha
human	IF	Glia; SSEA-4 and YKL-40 positive progenitor subtypes in the subventricular zone of developing human neocortex.; Authors - Christian B Brøchner
human	IHC	Genomics; MIN score predicts primary response to infliximab/adalimumab and vedolizumab therapy in patients with inflammatory bowel diseases.; Authors - Yuan Shi