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CHI3L1/YKL40 Polyclonal antibody

CHI3L1/YKL40 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 12036-1-AP


39 kDa synovial protein, ASRT7, Cartilage glycoprotein 39, CGP 39, CHI3L1, CHI3L1/YKL40, Chitinase 3 like protein 1, Chitinase 3-like 1, GP 39, GP39, HC gp39, hCGP 39, HCGP 3P, YKL 40, YKL40, YYL 40

Tested Applications

Positive WB detected inTHP-1 cells
Positive IHC detected inhuman liver tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12036-1-AP targets CHI3L1/YKL40 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHI3L1/YKL40 fusion protein Ag2710
Full Name chitinase 3-like 1 (cartilage glycoprotein-39)
Calculated Molecular Weight 383 aa, 43 kDa
Observed Molecular Weight 40, 33 kDa
GenBank Accession NumberBC008568
Gene Symbol CHI3L1
Gene ID (NCBI) 1116
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein. (PMID:21763261)


Product Specific Protocols
WB protocol for CHI3L1/YKL40 antibody 12036-1-APDownload protocol
IHC protocol for CHI3L1/YKL40 antibody 12036-1-APDownload protocol
IF protocol for CHI3L1/YKL40 antibody 12036-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



J Neurol Neurosurg Psychiatry

Molecules of senescent glial cells differentiate Alzheimer's disease from ageing

Authors - Linbin Dai
mouseIHC, IF, WB


Chitinase 3-like-1 contributes to acetaminophen-induced liver injury by promoting hepatic platelet recruitment.

Authors - Zhao Shan


MIN score predicts primary response to infliximab/adalimumab and vedolizumab therapy in patients with inflammatory bowel diseases.

Authors - Yuan Shi


SSEA-4 and YKL-40 positive progenitor subtypes in the subventricular zone of developing human neocortex.

Authors - Christian B Brøchner

Front Oncol

BYSL Promotes Glioblastoma Cell Migration, Invasion, and Mesenchymal Transition Through the GSK-3β/β-Catenin Signaling Pathway.

Authors - Zhuang Sha


Chitooligosaccharides Improve the Efficacy of Checkpoint Inhibitors in a Mouse Model of Lung Cancer.

Authors - Astrid Zedlitz Johansen