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- KD/KO Validated
CHI3L1/YKL40 Polyclonal antibody
CHI3L1/YKL40 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse and More (1)
WB, IHC, IF, ELISA
Cat no : 12036-1-AP
Validation Data Gallery
|Positive WB detected in||THP-1 cells|
|Positive IHC detected in||human liver tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|WB||See 4 publications below|
|IHC||See 6 publications below|
|IF||See 5 publications below|
12036-1-AP targets CHI3L1/YKL40 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CHI3L1/YKL40 fusion protein Ag2710|
|Full Name||chitinase 3-like 1 (cartilage glycoprotein-39)|
|Calculated molecular weight||383 aa, 43 kDa|
|Observed molecular weight||40, 33 kDa|
|GenBank accession number||BC008568|
|Gene ID (NCBI)||1116|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein. (PMID:21763261)
|Product Specific Protocols|
|WB protocol for CHI3L1/YKL40 antibody 12036-1-AP||Download protocol|
|IHC protocol for CHI3L1/YKL40 antibody 12036-1-AP||Download protocol|
|IF protocol for CHI3L1/YKL40 antibody 12036-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Neurol Neurosurg Psychiatry
Molecules of senescent glial cells differentiate Alzheimer's disease from ageing
Chitinase 3-like-1 contributes to acetaminophen-induced liver injury by promoting hepatic platelet recruitment.
MIN score predicts primary response to infliximab/adalimumab and vedolizumab therapy in patients with inflammatory bowel diseases.
SSEA-4 and YKL-40 positive progenitor subtypes in the subventricular zone of developing human neocortex.
BYSL Promotes Glioblastoma Cell Migration, Invasion, and Mesenchymal Transition Through the GSK-3β/β-Catenin Signaling Pathway.
Chitooligosaccharides Improve the Efficacy of Checkpoint Inhibitors in a Mouse Model of Lung Cancer.