CoraLite® Plus 488-conjugated CHI3L1/YKL40 Polyclonal antibody

• Featured Product
• KD/KO Validated

CHI3L1/YKL40 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-12036

Synonyms

39 kDa synovial protein, ASRT7, Cartilage glycoprotein 39, CGP 39, CHI3L1, Chitinase 3 like protein 1, DKFZp686N19119, FLJ38139, GP 39, GP39, HC gp39, hCGP 39, HCGP 3P, YKL 40, YKL40, YYL 40

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-12036

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 CHI3L1/YKL40 antibody (CL488-12036) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-12036 targets CHI3L1/YKL40 in IF applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHI3L1/YKL40 fusion protein Ag2710
• Full Name: chitinase 3-like 1 (cartilage glycoprotein-39)
• Calculated Molecular Weight: 383 aa, 43 kDa
• Observed Molecular Weight: 40, 33 kDa
• GenBank Accession Number: BC008568
• Gene Symbol: CHI3L1
• Gene ID (NCBI): 1116
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein. (PMID:21763261)