CHURC1 Polyclonal antibody

CHURC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12247-1-AP

Synonyms

C14orf52, chch, CHURC1, churchill domain containing 1, My015, Protein Churchill

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Validation Data Gallery

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  WB analysis of mouse skin using 12247-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 12247-1-AP (CHURC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12247-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12247-1-AP (CHURC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12247-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12247-1-AP (CHURC1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 12247-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12247-1-AP (CHURC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 12247-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12247-1-AP (CHURC1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human gliomas using 12247-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12247-1-AP (CHURC1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 12247-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12247-1-AP (CHURC1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 12247-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12247-1-AP (CHURC1 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse skin tissue, HeLa cells, mouse liver tissue
• Positive IHC detected in: human brain tissue, human gliomas tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12247-1-AP targets CHURC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CHURC1 fusion protein Ag2892
• Full Name: churchill domain containing 1
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC020550
• Gene Symbol: CHURC1
• Gene ID (NCBI): 91612
• RRID: AB_10666204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHURC1 (churchill domain containing 1), also known as C14orf52 or chch. The function of CHURC1 has not been widely studied, and is yet to be fully elucidated. It may be a transcriptional activator that mediates FGF signaling during neural development.The MW of this protein is 13 kDa, and Catalog#12247-1-AP specially recognises the 13 kDa protein.