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  • KD/KO Validated

CISD2 Polyclonal antibody

CISD2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 13318-1-AP

Synonyms

CDGSH iron sulfur domain 2, CDGSH2, CISD2, ERIS, Miner1, MitoNEET related 1 protein, NAF 1, WFS2, ZCD2



Tested Applications

Positive WB detected inmouse kidney tissue, mouse brain tissue, human brain tissue, HeLa cells, mouse heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue, human brain tissue, human lung tissue, human ovary tissue, human pancreas tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13318-1-AP targets CISD2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity Drosophila, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CISD2 fusion protein Ag4172
Full Name CDGSH iron sulfur domain 2
Calculated molecular weight 135 aa, 15 kDa
Observed molecular weight 13-15 kDa
GenBank accession numberBC032300
Gene symbol CISD2
Gene ID (NCBI) 493856
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum.

Protocols

Product Specific Protocols
WB protocol for CISD2 antibody 13318-1-APDownload protocol
IHC protocol for CISD2 antibody 13318-1-APDownload protocol
IF protocol for CISD2 antibody 13318-1-APDownload protocol
IP protocol for CISD2 antibody 13318-1-APDownload protocol
FC protocol for CISD2 antibody 13318-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Int J Clin Exp Pathol

CISD2 associated with proliferation indicates negative prognosis in patients with hepatocellular carcinoma.

Authors - Bin Chen
  • KD Validated
humanWB, IHC

Med Oncol

CISD2 expression is a novel marker correlating with pelvic lymph node metastasis and prognosis in patients with early-stage cervical cancer.

Authors - Luxin Liu
humanWB

BMC Med Genet

A donor splice site mutation in CISD2 generates multiple truncated, non-functional isoforms in Wolfram syndrome type 2 patients.

Authors - Monica Cattaneo
DrosophilaWB

Biol Open

Genetic studies in Drosophila and humans support a model for the concerted function of CISD2, PPT1 and CLN3 in disease.

Authors - Melanie A Jones
humanIHC,WB

Cancer Med

CISD2 enhances the chemosensitivity of gastric cancer through the enhancement of 5-FU-induced apoptosis and the inhibition of autophagy by AKT/mTOR pathway.

Authors - Yi Sun
humanWB

PLoS One

Dysfunction in the mitochondrial Fe-S assembly machinery leads to formation of the chemoresistant truncated VDAC1 isoform without HIF-1α activation.

Authors - Ioana Ferecatu