CISD2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CISD2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 13318-1-AP

Synonyms

MitoNEET related 1 protein, Miner1, ERIS, CDGSH2, CDGSH iron-sulfur domain-containing protein 2

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Validation Data Gallery

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  WB analysis using 13318-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13318-1-AP (CISD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13318-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13318-1-AP (CISD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13318-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13318-1-AP (CISD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 13318-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13318-1-AP (CISD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13318-1-AP

  IP result of anti-CISD2 (IP:13318-1-AP, 3ug; Detection:13318-1-AP 1:700) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human liver cancer using 13318-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13318-1-AP (CISD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13318-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13318-1-AP (CISD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13318-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CISD2 antibody (13318-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 13318-1-AP

  Immunofluorescent analysis of HepG2 cells using 13318-1-AP (CISD2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

• 

  FC experiment of HepG2 using 13318-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CISD2 Polyclonal antibody (13318-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse brain tissue, mouse heart tissue, human brain tissue, rat kidney tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 25 publications below
• IHC: See 11 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Product Information

13318-1-AP targets CISD2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, drosophila
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CISD2 fusion protein Ag4172
• Full Name: CDGSH iron sulfur domain 2
• Calculated Molecular Weight: 135 aa, 15 kDa
• Observed Molecular Weight: 13-15 kDa
• GenBank Accession Number: BC032300
• Gene Symbol: CISD2
• Gene ID (NCBI): 493856
• RRID: AB_2080270
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on the endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates the autophagy program by interacting with BCL2, contributing to antagonizing BECN1-mediated cellular autophagy at the endoplasmic reticulum.

Publications

Species	Application	Title
human, mouse, rat	WB	Proc Natl Acad Sci U S A; A calcium-dependent protease as a potential therapeutic target for Wolfram syndrome.; Authors - Simin Lu ; KD Validated
	WB	Elife; Sampling the conformational space of the catalytic subunit of human γ-secretase.; Authors - Xiao-Chen Bai
human	WB	EMBO Rep; Chemical targeting of NEET proteins reveals their function in mitochondrial morphodynamics.; Authors - Diana Molino ; KD Validated
human	WB,IHC	Oncotarget; Overexpressed CISD2 has prognostic value in human gastric cancer and promotes gastric cancer cell proliferation and tumorigenesis via AKT signaling pathway.; Authors - Lan Wang ; KD Validated
mouse	WB,IHC	FASEB J; A young testicular microenvironment protects Leydig cells against age-related dysfunction in a mouse model of premature aging.; Authors - Michael Curley ; KO Validated
human	WB,IHC	Oncotarget; A novel prognostic score model incorporating CDGSH Iron Sulfur Domain2 (CISD2) predicts risk of disease progression in laryngeal squamous cell carcinoma.; Authors - Lin Yang

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