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  • KD/KO Validated

CISD2 Polyclonal antibody

CISD2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 13318-1-AP

Synonyms

CDGSH iron sulfur domain 2, CDGSH2, CISD2, ERIS, Miner1, MitoNEET related 1 protein, NAF 1, WFS2, ZCD2



Tested Applications

Positive WB detected inmouse kidney tissue, mouse brain tissue, mouse heart tissue, human brain tissue, rat kidney tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

13318-1-AP targets CISD2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Drosophila
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CISD2 fusion protein Ag4172
Full Name CDGSH iron sulfur domain 2
Calculated molecular weight 135 aa, 15 kDa
Observed molecular weight 13-15 kDa
GenBank accession numberBC032300
Gene symbol CISD2
Gene ID (NCBI) 493856
RRIDAB_2080270
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum.

Protocols

Product Specific Protocols
WB protocol for CISD2 antibody 13318-1-APDownload protocol
IHC protocol for CISD2 antibody 13318-1-APDownload protocol
IF protocol for CISD2 antibody 13318-1-APDownload protocol
IP protocol for CISD2 antibody 13318-1-APDownload protocol
FC protocol for CISD2 antibody 13318-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human, mouse, ratWB

Proc Natl Acad Sci U S A

A calcium-dependent protease as a potential therapeutic target for Wolfram syndrome.

Authors - Simin Lu
  • KD Validated
WB

Elife

Sampling the conformational space of the catalytic subunit of human γ-secretase.

Authors - Xiao-Chen Bai
humanWB

EMBO Rep

Chemical targeting of NEET proteins reveals their function in mitochondrial morphodynamics.

Authors - Diana Molino
  • KD Validated
humanWB,IHC

Oncotarget

Overexpressed CISD2 has prognostic value in human gastric cancer and promotes gastric cancer cell proliferation and tumorigenesis via AKT signaling pathway.

Authors - Lan Wang
  • KD Validated
mouseWB,IHC

FASEB J

A young testicular microenvironment protects Leydig cells against age-related dysfunction in a mouse model of premature aging.

Authors - Michael Curley
  • KO Validated
humanWB,IHC

Oncotarget

A novel prognostic score model incorporating CDGSH Iron Sulfur Domain2 (CISD2) predicts risk of disease progression in laryngeal squamous cell carcinoma.

Authors - Lin Yang