- Featured Product
- KD/KO Validated
CISD2 Polyclonal antibody
CISD2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 13318-1-AP
Validation Data Gallery
|Positive WB detected in
|mouse kidney tissue, mouse brain tissue, mouse heart tissue, human brain tissue, rat kidney tissue
|Positive IP detected in
|mouse brain tissue
|Positive IHC detected in
|human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Positive FC detected in
|Western Blot (WB)
|WB : 1:2000-1:10000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
13318-1-AP targets CISD2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, Drosophila
|Host / Isotype
|Rabbit / IgG
|CISD2 fusion protein Ag4172
|CDGSH iron sulfur domain 2
|Calculated molecular weight
|135 aa, 15 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum.
|Product Specific Protocols
|WB protocol for CISD2 antibody 13318-1-AP
|IHC protocol for CISD2 antibody 13318-1-AP
|IF protocol for CISD2 antibody 13318-1-AP
|IP protocol for CISD2 antibody 13318-1-AP
|FC protocol for CISD2 antibody 13318-1-AP
|Click here to view our Standard Protocols
Proc Natl Acad Sci U S A
A calcium-dependent protease as a potential therapeutic target for Wolfram syndrome.
Sampling the conformational space of the catalytic subunit of human γ-secretase.
Chemical targeting of NEET proteins reveals their function in mitochondrial morphodynamics.
Overexpressed CISD2 has prognostic value in human gastric cancer and promotes gastric cancer cell proliferation and tumorigenesis via AKT signaling pathway.
A young testicular microenvironment protects Leydig cells against age-related dysfunction in a mouse model of premature aging.
A novel prognostic score model incorporating CDGSH Iron Sulfur Domain2 (CISD2) predicts risk of disease progression in laryngeal squamous cell carcinoma.