Validation Data Gallery
|Positive WB detected in||A431 cells, rat kidney tissue, HeLa cells, HepG2 cells, NIH/3T3 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human kidney tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16686-1-AP targets CKAP4 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CKAP4 fusion protein Ag10019|
|Full Name||cytoskeleton-associated protein 4|
|Calculated molecular weight||602 aa, 66 kDa|
|Observed molecular weight||63 kDa|
|GenBank accession number||BC082972|
|Gene ID (NCBI)||10970|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CKAP4, also named as Climp-63, is a 63 kDa cytoskeleton-linking membrane protein. It is the partner of triadin. CKAP4 is responsible for this association of triads and microtubules.
|Product Specific Protocols|
|WB protocol for CKAP4 antibody 16686-1-AP||Download protocol|
|IHC protocol for CKAP4 antibody 16686-1-AP||Download protocol|
|IF protocol for CKAP4 antibody 16686-1-AP||Download protocol|
|IP protocol for CKAP4 antibody 16686-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
WEI (Verified Customer) (06-28-2020)
Single strong band at correct size.