|Positive WB detected in||HepG2 cells, 3T3-L1 cells, HeLa cells, rat kidney tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human kidney tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
16686-1-AP targets CKAP4 in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CKAP4 fusion protein Ag10019|
|Full Name||cytoskeleton-associated protein 4|
|Calculated molecular weight||602 aa, 66 kDa|
|Observed molecular weight||63 kDa|
|GenBank accession number||BC082972|
|Gene ID (NCBI)||10970|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CKAP4, also named as Climp-63, is a 63 kDa cytoskeleton-linking membrane protein. It is the partner of triadin. CKAP4 is responsible for this association of triads and microtubules.
|Product Specific Protocols|
|WB protocol for CKAP4 antibody 16686-1-AP||Download protocol|
|IHC protocol for CKAP4 antibody 16686-1-AP||Download protocol|
|IF protocol for CKAP4 antibody 16686-1-AP||Download protocol|
|IP protocol for CKAP4 antibody 16686-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Proc Natl Acad Sci U S A
SENCR stabilizes vascular endothelial cell adherens junctions through interaction with CKAP4.
J Proteome Res
Significant Down-Regulation of Urea Cycle Generates Clinically Relevant Proteomic Signature in Hepatocellular Carcinoma Patients with Macrovascular Invasion.
Cell Death Dis
The RBP1-CKAP4 axis activates oncogenic autophagy and promotes cancer progression in oral squamous cell carcinoma.
Onco Targets Ther
MicroRNA-671-3p promotes proliferation and migration of glioma cells via targeting CKAP4.
Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.
J Biol Chem
Excessive ER-phagy mediated by the autophagy receptor FAM134B results in ER stress, the unfolded protein response, and cell death in HeLa cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
WEI (Verified Customer) (06-28-2020)
Single strong band at correct size.