CKAP4 Polyclonal antibody

CKAP4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16686-1-AP

Synonyms

63 kDa membrane protein, CKAP4, CLIMP 63, ERGIC 63, p63



Tested Applications

Positive WB detected inA431 cells, rat kidney tissue, HepG2 cells, HeLa cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16686-1-AP targets CKAP4 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CKAP4 fusion protein Ag10019
Full Name cytoskeleton-associated protein 4
Calculated molecular weight 602 aa, 66 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC082972
Gene symbol CKAP4
Gene ID (NCBI) 10970
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CKAP4, also named as Climp-63, is a 63 kDa cytoskeleton-linking membrane protein. It is the partner of triadin. CKAP4 is responsible for this association of triads and microtubules.

Protocols

Product Specific Protocols
WB protocol for CKAP4 antibody 16686-1-APDownload protocol
IHC protocol for CKAP4 antibody 16686-1-APDownload protocol
IF protocol for CKAP4 antibody 16686-1-APDownload protocol
IP protocol for CKAP4 antibody 16686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

Regulation of endoplasmic reticulum turnover by selective autophagy.

Authors - Aliaksandr Khaminets
humanWB

Mol Cell

Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.

Authors - Michael L van de Weijer
mouseIF

EMBO J

ER-to-lysosome-associated degradation of proteasome-resistant ATZ polymers occurs via receptor-mediated vesicular transport.

Authors - Ilaria Fregno
IF

EMBO J

MiT/TFE factors control ER-phagy via transcriptional regulation of FAM134B.

Authors - Laura Cinque
humanWB

EMBO J

CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.

Authors - Thaddaeus Mutugi Nthiga
humanIF

EMBO J

N-glycan processing selects ERAD-resistant misfolded proteins for ER-to-lysosome-associated degradation.

Authors - Ilaria Fregno

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

WEI (Verified Customer) (06-28-2020)

Single strong band at correct size.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Mouse Heart