CKAP4 Polyclonal antibody

CKAP4 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 16686-1-AP

Synonyms

63 kDa membrane protein, CKAP4, CLIMP 63, ERGIC 63, p63



Tested Applications

Positive WB detected inHepG2 cells, 3T3-L1 cells, HeLa cells, rat kidney tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

16686-1-AP targets CKAP4 in WB, IP, IHC, IF, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CKAP4 fusion protein Ag10019
Full Name cytoskeleton-associated protein 4
Calculated molecular weight 602 aa, 66 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC082972
Gene symbol CKAP4
Gene ID (NCBI) 10970
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CKAP4, also named as Climp-63, is a 63 kDa cytoskeleton-linking membrane protein. It is the partner of triadin. CKAP4 is responsible for this association of triads and microtubules.

Protocols

Product Specific Protocols
WB protocol for CKAP4 antibody 16686-1-APDownload protocol
IHC protocol for CKAP4 antibody 16686-1-APDownload protocol
IF protocol for CKAP4 antibody 16686-1-APDownload protocol
IP protocol for CKAP4 antibody 16686-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIP

Proc Natl Acad Sci U S A

SENCR stabilizes vascular endothelial cell adherens junctions through interaction with CKAP4.

Authors - Qing Lyu
humanWB

J Proteome Res

Significant Down-Regulation of Urea Cycle Generates Clinically Relevant Proteomic Signature in Hepatocellular Carcinoma Patients with Macrovascular Invasion.

Authors - Yin Cao
humanIHC,CoIP

Cell Death Dis

The RBP1-CKAP4 axis activates oncogenic autophagy and promotes cancer progression in oral squamous cell carcinoma.

Authors - Ling Gao
humanWB,IHC

Onco Targets Ther

MicroRNA-671-3p promotes proliferation and migration of glioma cells via targeting CKAP4.

Authors - Gui-Feng Lu
mouseWB,IP

Sci Rep

Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.

Authors - Tomoyuki Yamanaka
humanWB

J Biol Chem

Excessive ER-phagy mediated by the autophagy receptor FAM134B results in ER stress, the unfolded protein response, and cell death in HeLa cells.

Authors - Yangjie Liao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

WEI (Verified Customer) (06-28-2020)

Single strong band at correct size.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Mouse Heart