Validation Data Gallery
|Positive WB detected in||A431 cells, rat kidney tissue, HepG2 cells, HeLa cells, NIH/3T3 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human kidney tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:20000-1:100000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16686-1-AP targets CKAP4 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CKAP4 fusion protein Ag10019|
|Full Name||cytoskeleton-associated protein 4|
|Calculated molecular weight||602 aa, 66 kDa|
|Observed molecular weight||63 kDa|
|GenBank accession number||BC082972|
|Gene ID (NCBI)||10970|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CKAP4, also named as Climp-63, is a 63 kDa cytoskeleton-linking membrane protein. It is the partner of triadin. CKAP4 is responsible for this association of triads and microtubules.
|Product Specific Protocols|
|WB protocol for CKAP4 antibody 16686-1-AP||Download protocol|
|IHC protocol for CKAP4 antibody 16686-1-AP||Download protocol|
|IF protocol for CKAP4 antibody 16686-1-AP||Download protocol|
|IP protocol for CKAP4 antibody 16686-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Regulation of endoplasmic reticulum turnover by selective autophagy.
Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.
ER-to-lysosome-associated degradation of proteasome-resistant ATZ polymers occurs via receptor-mediated vesicular transport.
MiT/TFE factors control ER-phagy via transcriptional regulation of FAM134B.
CALCOCO1 acts with VAMP-associated proteins to mediate ER-phagy.
N-glycan processing selects ERAD-resistant misfolded proteins for ER-to-lysosome-associated degradation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
WEI (Verified Customer) (06-28-2020)
Single strong band at correct size.