Anti-Mouse CLEC12A/CD371 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using 98357-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CLEC12A/CD371 Rabbit RecAb (98357-1-RR, Clone: 242620B12) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 405 Anti-Mouse CD11c (N418) Mouse IgG2a Recombinant Antibody (CL405-65602, Clone: N418) and APC Anti-Mouse CD8a (53-6.7) (APC-65069, Clone: 53-6.7). CD8+ cells were gated. Cells were not fixed.

CLEC12A/CD371 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98357-1-RR

Clone No.242620B12

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD371, Clec12a, CLL-1, C-type lectin domain family 12 member A, C-type lectin-like molecule 1

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98357-1-RR targets CLEC12A/CD371 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2726

  Product name: Recombinant Mouse CLEC12A protein (rFc Tag) (HPLC verified)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 65-267 aa of /

  Sequence: YTTLATEMIKSNQLQRAKEELQENVFLQLKHNLNSSKKIKNLSAMLQSTATQLCRELYSKEPEHKCKPCPKGSEWYKDSCYSQLNQYGTWQESVMACSARNASLLKVKNKDVLEFIKYKKLRYFWLALLPRKDRTQYPLSEKMFLSEESERSTDDIDKKYCGYIDRVNVYYTYCTDENNIICEETASKVQLESVLNGLPEDSR

• Full Name: C-type lectin domain family 12, member a
• Calculated Molecular Weight: 31 kDa
• GenBank Accession Number: /
• Gene Symbol: Clec12a
• Gene ID (NCBI): 232413
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q504P2
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CLEC12A, also known as CD371, MICL, DCAL-2 or CLL-1, is a type II transmembrane glycoprotein and a member of the C-type lectin superfamily (PMID: 30401586). This protein, which contains a single C-type lectin-like domain and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM), is related to LOX-1 and Dectin-1 and is variably spliced and highly N-glycosylated (PMID: 14739280). Its ITIM sequence recruits the tyrosine phosphatases SHP-1 and SHP-2 to negatively regulate Syk signaling.