CLIC1 Recombinant antibody

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  WB analysis using 85831-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85831-4-RR (CLIC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85831-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85831-4-RR (CLIC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 85831-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85831-4-RR (CLIC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat spleen using 85831-4-RR

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 85831-4-RR (CLIC1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 85831-4-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CLIC1 antibody (85831-4-RR, Clone: 250143E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

CLIC1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 85831-4-RR

Clone No.250143E11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

G6, NCC27

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Tested Applications

• Positive WB detected in: HeLa cells, rat spleen tissue, HL-60 cells, C2C12 cells, RAW 264.7 cells, C6 cells, mouse spleen tissue
• Positive IHC detected in: mouse kidney tissue, rat spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85831-4-RR targets CLIC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CLIC1 fusion protein Ag6049
• Full Name: chloride intracellular channel 1
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27-32 kDa
• GenBank Accession Number: BC064527
• Gene Symbol: CLIC1
• Gene ID (NCBI): 1192
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00299
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chloride intracellular channel protein 1 (CLIC1) is a member of the chloride intracellular channel protein family. It plays a crucial role in various cellular processes, including the regulation of chloride ion transport, cell viability, and mitochondrial function. CLIC1 is involved in the formation of membrane-inserted channels that facilitate chloride ion movement, which is essential for maintaining cellular homeostasis and function (PMID: 32116799). Additionally, CLIC1 has been implicated in cancer progression, where it influences cell proliferation, migration, and invasion (PMID: 38027011).