|Positive WB detected in||human plasma tissue|
|Positive IHC detected in||human tonsillitis tissue, human breast cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12289-1-AP targets Clusterin in WB, IHC, CoIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Clusterin fusion protein Ag2889|
|Calculated molecular weight||449 aa, 53 kDa|
|Observed molecular weight||37 kDa|
|GenBank accession number||BC010514|
|Gene ID (NCBI)||1191|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).
Diabetes Metab Syndr Obes
Salidroside-Mitigated Inflammatory Injury of Hepatocytes with Non-Alcoholic Fatty Liver Disease via Inhibition TRPM2 Ion Channel Activation.
Int J Biometeorol
Bathing in carbon dioxide-enriched water alters protein expression in keratinocytes of skin tissue in rats.
Proteomic analysis of rat serum revealed the effects of chronic sleep deprivation on metabolic, cardiovascular and nervous system.
Predictive Nephrotoxicity Profiling of a Novel Antifungal Small Molecule in Comparison to Amphotericin B and Voriconazole.
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Expression of Clusterin suppresses Cr(VI)-induced premature senescence through activation of PI3K/AKT pathway.
Food Chem Toxicol
Clusterin overexpression as a potential neuroprotective response to the pathological effects of high fat dieting on the brain reward system.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
George (Verified Customer) (12-03-2018)
IHC performed on paraffin-embedded human kidney tissue