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CoraLite®488-conjugated CNOT1 Monoclonal antibody

CNOT1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

1A10A11

Cat no : CL488-66507

Synonyms

AD 005, CCR4 associated factor 1, CDC39, CNOT1, DKFZp686E0722, DKFZp686O168, FLJ36492, FLJ90644, hNOT1, KIAA1007, NOT1, NOT1H



Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66507-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-66507 targets CNOT1 in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CNOT1 fusion protein Ag5623
Full Name CCR4-NOT transcription complex, subunit 1
Calculated molecular weight 267 kDa
Observed molecular weight 240-250 kDa
GenBank accession numberBC040523
Gene symbol CNOT1
Gene ID (NCBI) 23019
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission maxima wavelengths488 nm/515 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms. CNOT1 exsits as many isoforms. The isoform one is about 267 kDa and the isoform four is about 173 kDa.

Protocols

Product Specific Protocols
IF protocol for CL488 CNOT1 antibody CL488-66507Download protocol
Standard Protocols
Click here to view our Standard Protocols