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CNOT1 Monoclonal antibody

CNOT1 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A10A11

Cat no : 66507-1-Ig

Synonyms

AD 005, CCR4 associated factor 1, CDC39, CNOT1, hNOT1, KIAA1007, NOT1, NOT1H



Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, human cerebellum tissue, pig kidney tissue, mouse kidney tissue, HeLa cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66507-1-Ig targets CNOT1 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CNOT1 fusion protein Ag5623
Full Name CCR4-NOT transcription complex, subunit 1
Calculated molecular weight 267 kDa
Observed molecular weight 240-250 kDa
GenBank accession numberBC040523
Gene symbol CNOT1
Gene ID (NCBI) 23019
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms. CNOT1 exsits as many isoforms. The isoform one is about 267 kDa and the isoform four is about 173 kDa.

Protocols

Product Specific Protocols
WB protocol for CNOT1 antibody 66507-1-IgDownload protocol
IHC protocol for CNOT1 antibody 66507-1-IgDownload protocol
IF protocol for CNOT1 antibody 66507-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

Argonaute-miRNA Complexes Silence Target mRNAs in the Nucleus of Mammalian Stem Cells.

Authors - Aishe A Sarshad
  • KD Validated