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- KD/KO Validated
CNOT1 Monoclonal antibody
CNOT1 Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 66507-1-Ig
Validation Data Gallery
|Positive WB detected in||U2OS cells, A549 cells, HEK-293 cells, human cerebellum tissue, pig kidney tissue, mouse kidney tissue, HeLa cells, LNCaP cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells|
|Positive IHC detected in||human heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66507-1-Ig targets CNOT1 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
|Tested Reactivity||Human, mouse, rat, pig|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||CNOT1 fusion protein Ag5623|
|Full Name||CCR4-NOT transcription complex, subunit 1|
|Calculated molecular weight||267 kDa|
|Observed molecular weight||240-250 kDa|
|GenBank accession number||BC040523|
|Gene ID (NCBI)||23019|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms. CNOT1 exsits as many isoforms. The isoform one is about 267 kDa and the isoform four is about 173 kDa.
Argonaute-miRNA Complexes Silence Target mRNAs in the Nucleus of Mammalian Stem Cells.
TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.
The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation