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  • KD/KO Validated

CNOT1 Monoclonal antibody

CNOT1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


Human, mouse, rat, pig and More (1)







Cat no : 66507-1-Ig


AD 005, CCR4 associated factor 1, CDC39, CNOT1, hNOT1, KIAA1007, NOT1, NOT1H

Tested Applications

Positive WB detected inU2OS cells, A549 cells, HEK-293 cells, human cerebellum tissue, pig kidney tissue, mouse kidney tissue, HeLa cells, LNCaP cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66507-1-Ig targets CNOT1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CNOT1 fusion protein Ag5623
Full Name CCR4-NOT transcription complex, subunit 1
Calculated Molecular Weight 267 kDa
Observed Molecular Weight 240-250 kDa
GenBank Accession NumberBC040523
Gene Symbol CNOT1
Gene ID (NCBI) 23019
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CNOT1 is a component of CCR4-NOT protein complex, which is a regulator of RNA polymerase II transcription, acts as a transcription repressor. CCR4-NOT complex could participate in transcription as well as mRNA degradation. It's highly expressed in brain, heart, thymus, but weak in skeletal muscle and colon. CNOT1 undergoes alternative splicing to produce four isoforms. CNOT1 exsits as many isoforms. The isoform one is about 267 kDa and the isoform four is about 173 kDa.


Product Specific Protocols
WB protocol for CNOT1 antibody 66507-1-IgDownload protocol
IHC protocol for CNOT1 antibody 66507-1-IgDownload protocol
IF protocol for CNOT1 antibody 66507-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Cell

Argonaute-miRNA Complexes Silence Target mRNAs in the Nucleus of Mammalian Stem Cells.

Authors - Aishe A Sarshad
  • KD Validated

Nat Commun

TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.

Authors - Roy Matkovic

Cell Rep

The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation

Authors - Nicolas Curdy


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Roy (Verified Customer) (06-12-2024)

Great antibody to detect CNOT1 by WB from HeLa nuclear and cytoplasmic extracts (1/1000 - Overnight incubation at 4°C). Does also detect the cytoplasmic CNOT1 by immunofluorescence (1/250) in PFA 4% fixed HeLa cells.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1/1000 (WB) - 1/250 (IF)
  • Cell Tissue Type: HeLa