Cannabinoid receptor 1 Polyclonal antibody

Cannabinoid receptor 1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 17978-1-AP

Synonyms

CANN6, Cannabinoid receptor 1, cannabinoid receptor 1 (brain), CB R, CB1, CB1A, CB1K5, CB1R, CNR, CNR1

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Validation Data Gallery

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  WB analysis using 17978-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17978-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17978-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17978-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17978-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17978-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 17978-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17978-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17978-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17978-1-AP (Cannabinoid receptor 1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 17978-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Cannabinoid receptor 1 antibody (17978-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of SH-SY5Y using 17978-1-AP

  1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human Cannabinoid receptor 1 (17978-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue
• Positive FC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Product Information

17978-1-AP targets Cannabinoid receptor 1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cannabinoid receptor 1 fusion protein Ag12371
• Full Name: cannabinoid receptor 1 (brain)
• Calculated Molecular Weight: 472 aa, 53 kDa
• Observed Molecular Weight: 45 kDa, 66 kDa
• GenBank Accession Number: BC074812
• Gene Symbol: CNR1
• Gene ID (NCBI): 1268
• RRID: AB_10859098
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cannabinoid receptor 1 (CNR1, or CB1) and CNR2 (CB2) are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family. The CB1 receptor is expressed mainly in the brain. The CB2 receptor is expressed mainly in the immune system and in hematopoietic cells. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana.

Publications

Species	Application	Title
mouse	WB	Elife; p21-activated kinase 1 restricts tonic endocannabinoid signaling in the hippocampus.; Authors - Shuting Xia
mouse	WB	Chin Med; Electroacupuncture repairs intestinal barrier by upregulating CB1 through gut microbiota in DSS-induced acute colitis; Authors - Jingze Yang
mouse	WB	Hum Mol Genet; BDNF overexpression prevents cognitive deficit elicited by adolescent cannabis exposure and host susceptibility interaction.; Authors - Hadar Segal-Gavish
rat	IHC	Phytomedicine; Endocannabinoid signalling/cannabinoid receptor 2 is involved in icariin-mediated protective effects against bleomycin-induced pulmonary fibrosis.; Authors - Wenjing Du
rat	WB	Food Funct; L-Theanine regulates glutamine metabolism and immune function by binding to cannabinoid receptor 1.; Authors - An Liu
mouse,rat	WB,IHC,IF	Int J Cardiol; Indoxyl sulfate upregulates the cannabinoid type 1 receptor gene via an ATF3/c-Jun complex-mediated signaling pathway in the model of uremic cardiomyopathy.; Authors - Yu-Juei Hsu

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