CNTF Polyclonal antibody

CNTF Polyclonal Antibody for IF, IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, IF, Cell treatment, ELISA

Conjugate

Unconjugated

Cat no : 27342-1-AP

Synonyms

ciliary neurotrophic factor, CNTF, HCNTF



Tested Applications

Positive IHC detected inhuman cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-12 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 27342-1-AP is CNTF Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CNTF fusion protein Ag26410
Full Name ciliary neurotrophic factor
Calculated molecular weight 200 aa, 23 kDa
GenBank accession numberBC074964
Gene symbol CNTF
Gene ID (NCBI) 1270
RRIDAB_2880848
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for CNTF antibody 27342-1-APDownload protocol
IF protocol for CNTF antibody 27342-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCell treatment

Transl Psychiatry

Chronic cortisol differentially impacts stem cell-derived astrocytes from major depressive disorder patients.

Authors - Kelly J Heard
mouseIF

bioRxiv

Lipid nanoparticle-mediated delivery of mRNA into the mouse and human retina and other ocular tissues

Authors - Cheri Z Chambers