CNTF Polyclonal antibody

CNTF Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 27342-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

ciliary neurotrophic factor, CNTF, HCNTF

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive IHC detected inhuman cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27342-1-AP targets CNTF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26410

Product name: Recombinant human CNTF protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-80 aa of BC074964

Sequence: MAFTEHSPLTPHRRDLCSRSIWLARKIRSDLTALTESYVKHQGLNKNINLDSADGMPVASTDQWSELTEAERLQENLQAY

Predict reactive species
Full Name ciliary neurotrophic factor
Calculated Molecular Weight 200 aa, 23 kDa
GenBank Accession NumberBC074964
Gene Symbol CNTF
Gene ID (NCBI) 1270
RRIDAB_2880848
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP26441
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for CNTF antibody 27342-1-APDownload protocol
IF protocol for CNTF antibody 27342-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

bioRxiv

Lipid nanoparticle-mediated delivery of mRNA into the mouse and human retina and other ocular tissues

Authors - Cheri Z Chambers
mouseIF

Transl Vis Sci Technol

Lipid Nanoparticle-Mediated Delivery of mRNA Into the Mouse and Human Retina and Other Ocular Tissues

Authors - Cheri Z Chambers
humanWB,IHC

J Mol Histol

Ciliary neurotrophic factor (CNTF) contributes to pelvic organ prolapse by modulating collagen expression via the JAK2-STAT3 pathway

Authors - Zhonglei Xu
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