Collagen Type XVII Polyclonal antibody, PBS Only

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• Collagen Type XVII Polyclonal antibody, PBS Only (31190-1-PBS)

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  WB analysis using 31190-1-AP (same clone as 31190-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31190-1-AP (COL17A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skin using 31190-1-AP (same clone as 31190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31190-1-AP (COL17A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

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  IHC staining of rat skin using 31190-1-AP (same clone as 31190-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31190-1-AP (COL17A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 31190-1-AP (same clone as 31190-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using COL17A1 antibody (31190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

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  IF Staining of HaCaT using 31190-1-AP (same clone as 31190-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using COL17A1 antibody (31190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31190-1-PBS in a different storage buffer formulation.

Collagen Type XVII Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 31190-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

COL17, COL17A1, LABD97, BP180, Collagen alpha 1(XVII) chain

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Product Information

31190-1-PBS targets Collagen Type XVII in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34645

  Product name: Recombinant human COL17A1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-200 aa of BC168368

  Sequence: MDVTKKNKRDGTEVTERIVTETVTTRLTSLPPKGGTSNGYAKTASLGGGSRLEKQSLTHGSSGYINSTGSTRGHASTSSYRRAHSPASTLPNSPGSTFERKTHVTRHAYEGSSSGNSSPEYPRKEFASSSTRGRSQTRESEIRVRLQSASPSTRWTELDDVKRLLKGSRSASVSPTRNSSNTLPIPKKGTVETKIVTASS

• Full Name: collagen, type XVII, alpha 1
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC168368
• Gene Symbol: COL17A1
• Gene ID (NCBI): 1308
• RRID: AB_3669890
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9UMD9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Collagen alpha-1(XVII) chain, also termed 180-kDa bullous pemphigoid antigen (BP180), is a type II transmembrane glycoprotein with a collagenous carboxyl-terminal extracellular domain and a noncollagenous amino-terminal cytoplasmic domain. High expression of COL17A1 has also been identified as a marker of human esophageal quiescent stem/progenitor cells (PMID: 35709398, 9545306). As a structural component of the hemidesmosome, COL17A1 is not only present as the full-length 180 kDa transmembrane form, but also as a cleaved 120 kDa soluble form that is further processed to a 97 kDa form (PMID: 113594550).