Collagen Type XVII Polyclonal antibody

Collagen Type XVII Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 31190-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

COL17, COL17A1, LABD97, BP180, Collagen alpha 1(XVII) chain

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllWBIF/ICC

WB analysis using 31190-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 31190-1-AP (COL17A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 31190-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 31190-1-AP (Collagen Type XVII antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of A431 using 31190-1-AP
WB result of Collagen Type XVII antibody (31190-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Collagen Type XVII transfected A431 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of A431 using 31190-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using COL17A1 antibody (31190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HaCaT using 31190-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using COL17A1 antibody (31190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	A431 cells, rat skin tissue, mouse skin tissue
Positive IF/ICC detected in	A431 cells, HaCaT cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31190-1-AP targets Collagen Type XVII in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag34645 Product name: Recombinant human COL17A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-200 aa of BC168368 Sequence: MDVTKKNKRDGTEVTERIVTETVTTRLTSLPPKGGTSNGYAKTASLGGGSRLEKQSLTHGSSGYINSTGSTRGHASTSSYRRAHSPASTLPNSPGSTFERKTHVTRHAYEGSSSGNSSPEYPRKEFASSSTRGRSQTRESEIRVRLQSASPSTRWTELDDVKRLLKGSRSASVSPTRNSSNTLPIPKKGTVETKIVTASS Predict reactive species
Full Name	collagen, type XVII, alpha 1
Observed Molecular Weight	180 kDa
GenBank Accession Number	BC168368
Gene Symbol	COL17A1
Gene ID (NCBI)	1308
RRID	AB_3669890
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity Purification
UNIPROT ID	Q9UMD9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Collagen alpha-1(XVII) chain, also termed 180-kDa bullous pemphigoid antigen (BP180), is a type II transmembrane glycoprotein with a collagenous carboxyl-terminal extracellular domain and a noncollagenous amino-terminal cytoplasmic domain. High expression of COL17A1 has also been identified as a marker of human esophageal quiescent stem/progenitor cells (PMID: 35709398, 9545306). As a structural component of the hemidesmosome, COL17A1 is not only present as the full-length 180 kDa transmembrane form, but also as a cleaved 120 kDa soluble form that is further processed to a 97 kDa form (PMID: 113594550).

Protocols

Product Specific Protocols
IF protocol for Collagen Type XVII antibody 31190-1-AP	Download protocol
WB protocol for Collagen Type XVII antibody 31190-1-AP	Download protocol

Standard Protocols
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Publications

What published studies show

The Collagen Type XVII (COL17A1) polyclonal antibody, catalog number 31190-1-AP, has 1 total citation. It appears in the Journal of Investigative Dermatology (2024), in a study demonstrating that Paxbp1 is indispensable for maintaining epidermal homeostasis in mouse skin. The research field is dermatology and epidermal biology, with the antibody applied via IHC on mouse tissue.