Collagen Type XVII Polyclonal antibody

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  WB analysis using 31190-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31190-1-AP (COL17A1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of rat skin using 31190-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31190-1-AP (COL17A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat skin using 31190-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 31190-1-AP (COL17A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 31190-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using COL17A1 antibody (31190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HaCaT using 31190-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using COL17A1 antibody (31190-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Collagen Type XVII Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 31190-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

COL17, COL17A1, LABD97, BP180, Collagen alpha 1(XVII) chain

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Tested Applications

• Positive WB detected in: A431 cells, mouse skin tissue
• Positive IHC detected in: rat skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells, HaCaT cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

31190-1-AP targets Collagen Type XVII in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34645

  Product name: Recombinant human COL17A1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-200 aa of BC168368

  Sequence: MDVTKKNKRDGTEVTERIVTETVTTRLTSLPPKGGTSNGYAKTASLGGGSRLEKQSLTHGSSGYINSTGSTRGHASTSSYRRAHSPASTLPNSPGSTFERKTHVTRHAYEGSSSGNSSPEYPRKEFASSSTRGRSQTRESEIRVRLQSASPSTRWTELDDVKRLLKGSRSASVSPTRNSSNTLPIPKKGTVETKIVTASS

• Full Name: collagen, type XVII, alpha 1
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC168368
• Gene Symbol: COL17A1
• Gene ID (NCBI): 1308
• RRID: AB_3669890
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9UMD9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Collagen alpha-1(XVII) chain, also termed 180-kDa bullous pemphigoid antigen (BP180), is a type II transmembrane glycoprotein with a collagenous carboxyl-terminal extracellular domain and a noncollagenous amino-terminal cytoplasmic domain. High expression of COL17A1 has also been identified as a marker of human esophageal quiescent stem/progenitor cells (PMID: 35709398, 9545306). As a structural component of the hemidesmosome, COL17A1 is not only present as the full-length 180 kDa transmembrane form, but also as a cleaved 120 kDa soluble form that is further processed to a 97 kDa form (PMID: 113594550).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	J Invest Dermatol; Paxbp1 is indispensable for the maintenance of epidermal homeostasis; Authors - Cong Huang