COTL1 Monoclonal antibody

COTL1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

4A3D9

Cat no : 60237-1-Ig

Synonyms

CLP, Coactosin like protein, COTL1



Tested Applications

Positive WB detected inA431 cells, fetal human brain tissue, human peripheral blood platelets
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60237-1-Ig is COTL1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COTL1 fusion protein Ag1230
Full Name coactosin-like 1 (Dictyostelium)
Calculated molecular weight 16 kDa
Observed molecular weight 14-16 kDa
GenBank accession numberBC010884
Gene symbol COTL1
Gene ID (NCBI) 23406
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.

Protocols

Product Specific Protocols
WB protocol for COTL1 antibody 60237-1-IgDownload protocol
IHC protocol for COTL1 antibody 60237-1-IgDownload protocol
IF protocol for COTL1 antibody 60237-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols