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CoraLite® Plus 488-conjugated COTL1 Monoclonal antibody

COTL1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4A3D9

Cat no : CL488-60237

Synonyms

CLP, Coactosin like protein, COTL1, FLJ43657

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated COTL1 Monoclonal ant (CL488-60237)

    IF Staining of HepG2 using CL488-60237

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488-conjugated COTL1 antibody (CL488-60237, Clone: 4A3D9 ) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated COTL1 Monoclonal ant (CL488-60237)

    IF Staining of HepG2 using CL488-60237

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 COTL1 antibody (CL488-60237, Clone: 4A3D9 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL488-60237 is COTL1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen COTL1 fusion protein Ag1230
Full Name coactosin-like 1 (Dictyostelium)
Calculated Molecular Weight 16 kDa
Observed Molecular Weight 16 kDa
GenBank Accession NumberBC010884
Gene Symbol COTL1
Gene ID (NCBI) 23406
RRIDAB_2883124
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 COTL1 antibody CL488-60237Download protocol
Standard Protocols
Click here to view our Standard Protocols