• Featured Product
  • KD/KO Validated

RAIDD Polyclonal antibody

RAIDD Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10401-1-AP

Synonyms

CRADD, RAIDD



Tested Applications

Positive WB detected inK-562 cells, HEK-293 cells, HeLa cells, MCF-7 cells, mouse heart tissue, mouse kidney tissue, mouse liver tissue, mouse skeletal muscle tissue, mouse testis tissue, Raji cells
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10401-1-AP targets RAIDD in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAIDD fusion protein Ag0630
Full Name CASP2 and RIPK1 domain containing adaptor with death domain
Calculated molecular weight 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC017042
Gene symbol CRADD
Gene ID (NCBI) 8738
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RAIDD, also named as CRADD, is an apoptotic adaptor molecule specific for caspase-2 and FASL/TNF receptor-interacting protein RIP. In the presence of RIP and TRADD, CRADD recruits caspase-2 to the TNFR-1 signalling complex. CRADD interacts with BCL10 through its caspase recruitment domain and suppresses interactions between BCL10 and CARMA1. It is a negative regulator of the CARMA1 signalosome and suppressor of Th1-and Th17-mediated inflammatory responses.

Protocols

Product Specific Protocols
WB protocol for RAIDD antibody 10401-1-APDownload protocol
IHC protocol for RAIDD antibody 10401-1-APDownload protocol
IP protocol for RAIDD antibody 10401-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human, mouseWB

J Biol Chem

The Adaptor CRADD/RAIDD Controls Activation of Endothelial Cells by Proinflammatory Stimuli.

Authors - Huan Qiao
mouseWB

J Immunol

Cutting edge: the "death" adaptor CRADD/RAIDD targets BCL10 and suppresses agonist-induced cytokine expression in T lymphocytes.

Authors - Lin Qing Q
  • KO Validated
mouseWB

EMBO Rep

PIDDosome-induced p53-dependent ploidy restriction facilitates hepatocarcinogenesis.

Authors - Valentina C Sladky
mouseWB

Dev Cell

E2F-Family Members Engage the PIDDosome to Limit Hepatocyte Ploidy in Liver Development and Regeneration.

Authors - Valentina C Sladky
  • KD Validated