CRX Monoclonal antibody

CRX Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C3C11

Cat no : 67555-1-Ig

Synonyms

cone rod homeobox, Cone rod homeobox protein, CORD2, CRD, CRX, LCA7, OTX3

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Validation Data Gallery

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  WB analysis using 67555-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67555-1-Ig (CRX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 67555-1-Ig

  Y79 cells were subjected to SDS PAGE followed by western blot with 67555-1-Ig (CRX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: rat retina tissue, Y79 cells, mouse retina tissue, pig retina tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

Sample-dependent, check data in validation data gallery

Product Information

67555-1-Ig targets CRX in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CRX fusion protein Ag30086
• Full Name: cone-rod homeobox
• Calculated molecular weight: 299 aa, 32 kDa
• Observed molecular weight: 37 kDa
• GenBank accession number: BC016664
• Gene symbol: CRX
• Gene ID (NCBI): 1406
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cone-rod homeobox (Crx) is a homeodomain transcription factor and member of the Otx family, which has been thought to play a critical role in determining and maintaining the phenotype of both pinealocytes and retinal photoreceptors [PMID:17467693]. In the mammalian retina, Crx plays an essential role in the normal development and maintenance of cones and rods and regulates expression of the network of genes that characterize the retina [PMID:17653270]. Elimination of Crx by disruption of the homeobox domain results in loss of the image-forming visual system but not the non-image-forming visual system controlling circadian rhythms [PMID:20438719].