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- KD/KO Validated
CST1 Polyclonal antibody
CST1 Polyclonal Antibody for WB, FC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IF, FC, IHC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 16025-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | human saliva tissue, human testis tissue, COLO 320 cells, human adrenal gland tissue |
Positive FC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 9 publications below |
IHC | See 9 publications below |
IF | See 2 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Product Information
16025-1-AP targets CST1 in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | CST1 fusion protein Ag8862 |
Full Name | cystatin SN |
Calculated Molecular Weight | 141 aa, 16 kDa |
Observed Molecular Weight | 14-16 kDa |
GenBank Accession Number | BC021225 |
Gene Symbol | CST1 |
Gene ID (NCBI) | 1469 |
RRID | AB_10916385 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CST1 antibody 16025-1-AP | Download protocol |
FC protocol for CST1 antibody 16025-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Rep Med Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application | ||
Nat Commun Integrated single-cell transcriptome analysis reveals heterogeneity of esophageal squamous cell carcinoma microenvironment. | ||
Cell Res Differential secretome analysis reveals CST6 as a suppressor of breast cancer bone metastasis. | ||
Oncogene CST1 inhibits ferroptosis and promotes gastric cancer metastasis by regulating GPX4 protein stability via OTUB1
| ||
Mol Oncol Cancer-associated fibroblasts educate normal fibroblasts to facilitate cancer cell spreading and T cell suppression. | ||
Aging (Albany NY) Transcriptional dysregulation of TRIM29 promotes colorectal cancer carcinogenesis via pyruvate kinase-mediated glucose metabolism. |