Cystatin SN/CST1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Cystatin SN/CST1 Polyclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 16025-1-AP

Synonyms

CST1, Cystatin-1, cystatin SN, Cystatin 1, Cystain-SA-I

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Validation Data Gallery

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  WB analysis of human saliva using 16025-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 16025-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 16025-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human adrenal gland using 16025-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 16025-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16025-1-AP (CST1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  FC experiment of MCF-7 using 16025-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human CST1 (16025-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: MDA-MB-231 cells, COLO 320 cells, human adrenal gland tissue, human testis tissue, human saliva, SW480 cells, mouse skin tissue
• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 9 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Product Information

16025-1-AP targets Cystatin SN/CST1 in WB, IHC, IF, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cystatin SN/CST1 fusion protein Ag8862
• Full Name: cystatin SN
• Calculated Molecular Weight: 141 aa, 16 kDa
• Observed Molecular Weight: 14-16 kDa
• GenBank Accession Number: BC021225
• Gene Symbol: CST1
• Gene ID (NCBI): 1469
• RRID: AB_10916385
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CST1, or cystatin SN, is a gene in humans that encodes a secreted protein belonging to the type 2 cystatin superfamily, which includes CST1, CST2, CST3, CST4, and CST5. These proteins are cysteine proteinase inhibitors found in various human fluids and secretions, where they appear to provide protective functions . CST1 is specifically found in saliva, tears, urine, and seminal fluid, and it plays a role in inhibiting the activity of cysteine proteases. CST1 has been implicated in various pathological processes, including tumor invasion and metastasis. Overexpression of CST1 has been observed in several types of cancer, such as lung, breast, colorectal, and gastric cancer, suggesting its role in the proliferation, invasion, and metastasis of these tumors.

Publications

Species	Application	Title
human	IHC	Cell Rep Med; Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application; Authors - Xiaoxue Ren
human	IHC,IF	Nat Commun; Integrated single-cell transcriptome analysis reveals heterogeneity of esophageal squamous cell carcinoma microenvironment.; Authors - Huy Q Dinh
human	WB	Cell Res; Differential secretome analysis reveals CST6 as a suppressor of breast cancer bone metastasis.; Authors - Jin Lei L
human	WB,IHC,IF,IP	Oncogene; CST1 inhibits ferroptosis and promotes gastric cancer metastasis by regulating GPX4 protein stability via OTUB1; Authors - Dongbao Li ; KD Validated
human	WB	Mol Oncol; Cancer-associated fibroblasts educate normal fibroblasts to facilitate cancer cell spreading and T cell suppression.; Authors - Go Itoh
human	IHC	Aging (Albany NY); Transcriptional dysregulation of TRIM29 promotes colorectal cancer carcinogenesis via pyruvate kinase-mediated glucose metabolism.; Authors - Jing Han